Anti-Mouse EphA6

Anti-Mouse EphA6

Product No.: E264

[product_table name="All Top" skus="E264"]

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Target
EphA6
Product Type
Polyclonal Antibody
Alternate Names
Ephrin Receptor 6, Ehk2, Hek12, M-ehk2, DKFZp434C1418, EPA6, FLJ35246, PRO57066
Applications
WB

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Goat
Immunogen
NS0 - Derived Recombinant Mouse EphA6 Extracellular Domain
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Mouse EphA6 this polyclonal antibody can be used at a concentration of 0.1 - 0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for rmEphA6 is approximately 10 ng/lane and 5 ng/lane under non-reducingand reducing conditions, respectively. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Mouse EphA6 recognizes Mouse EphA6. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Ephrin receptor (EphA6) is a receptor1 that is the same as EHK2 (EPH homology kinase-2)2 which is expressed almost exclusively in nervous system. The receptor is expressed in cochlear ganglion cells.1 EphA6 is involved in neural circuits underlying aspects of learning and memory.3

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Lee, AM. et al. (1996) DNA and Cell Biol. 15: 817
2. Maisonpierre, PC. et al. (1997) Oncogene 8: 32777
3. Lanthorn, TH. et al. (2008) Neurosci Lett. 438: 205
General Western Blot Protocol

Certificate of Analysis

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Prod No.
Description
E264
E214
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.