Anti-TrpC5 Ca+2 Channel Antibody (11541)
Anti-TrpC5 Ca+2 Channel Antibody (11541)
Product No.: 11541
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Clone S67-15 Target TrpC5 Ca+2 Channel Formats AvailableView All Product Type Monoclonal Alternate Names TrpC5, Transient receptor protein 5, TRP-5, hTRP-5, hTRP5 Isotype Mouse IgG2b Applications IHC , IP , WB , AM |
Data
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TrpC5 Monoclonal Antibody, Clone N67/15 (11541). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TrpC5 Monoclonal Antibody (11541) at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TrpC5 Antibody (D) Composite.
Immunohistochemistry analysis using Mouse Anti-TrpC5 Monoclonal Antibody, Clone N67/15 (11541). Tissue: Brain Slice. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-TrpC5 Monoclonal Antibody (11541) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 µl for 5 minutes at RT. Localization: Nuclear staining. Magnification: 10X.
Western Blot analysis of Mouse brain showing detection of 110 kDa TrpC5 protein using Mouse Anti-TrpC5 Monoclonal Antibody, Clone N67/15 (11541). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-TrpC5 Monoclonal Antibody (11541) at 1:1000 for Overnight at 4C. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:7000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: 110 kDa. - -
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Antibody DetailsProduct DetailsReactivity Species Human ⋅ Mouse ⋅ Rat Host Species Mouse Immunogen Synthetic peptide corresponding to aa 827-845 of human TrpC5 (accession no. Q9UL62). Product Concentration Lot Specific Formulation PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C. Avoid repeated freeze-thaw cycles. Regulatory Status For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1-10ug/mL. A band of ~110kDa is detected.Immunohistochemistry and
Immunocytochemistry: use at 0.1-1ug/mL Immunofluorescence: use at 1-10ug/mL These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain lysate. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This antibody recognizes human, mouse,
and rat TrpC5. Background Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. TrpC5 (transient receptor potential cation channel, subfamily C, member 5) is a subtype of the TrpC family of mammalian transient receptor potential ion channels. Homomultimeric TrpC5 and heteromultimeric TrpC5-TrpC1 channels are activated by extracellular reduced thioredoxin. This activation has been associated with rheumatoid arthritis as well as with action on anesthetics such as chloroform, halothane, and propofol. Antigen DetailsFunction Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol sUniProtKB:Q9QX29, PubMed:16284075}. NCBI Gene Bank ID UniProt.org Research Area Ion Channels References & CitationsTechnical Protocols   AM |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
