Anti-CKIIα’ Antibody (13032)
Data
Detection of human CKII alpha’ by immunohistochemistry. 
Detection of human and mouse CKII alpha’ by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CKII alpha’ antibody 13032 (lot 13032-5) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. - -
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Antibody DetailsProduct DetailsReactivity Species Human Host Species Rabbit Immunogen Synthetic peptide representing a portion of the protein located within exon 11 (LocusLink ID 1459). Product Concentration Lot Specific Formulation Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide State of Matter Liquid Product Preparation Purified by immunoaffinity chromatography Storage and Handling Store at 2 - 8°C. Antibody is stable at 2 - 8°C for 1 year. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Western Blot: 0.2 - 1 ug/ml.
Immunoprecipitation: 4-10 ug/mg lysate. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rabbit Polyclonal Antibody specific to human CKII α, other species have not been tested. Antigen DetailsFunction Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response. During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation. Can also negatively regulate apoptosis. Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3. Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8. Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV. Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB. Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function. Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1. Acts as an ectokinase that phosphorylates several extracellular proteins. During viral infection, phosphorylates various proteins involved in the viral life cycles of EBV, HSV, HBV, HCV, HIV, CMV and HPV. {PubMed:11239457, PubMed:11704824, PubMed:16193064}. NCBI Gene Bank ID UniProt.org Research Area Enzymes References & CitationsTechnical Protocols  |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
