Anti-Angiotensin Converting Enzyme 2 (ACE2) (19506)

Anti-Angiotensin Converting Enzyme 2 (ACE2) (19506)

Product No.: 19506

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Target
Angiotensin Converting Enzyme 2 (ACE2)
Product Type
Polyclonal
Isotype
Rabbit
Applications
IHC

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Select Product Size

Data

Immunohistochemistry: use at dilution of 1:25-1:100. Detection of ACE2 in paraffin-embedded human thyroid carcinoma with #19506 diluted 1:30.
Immunohistochemistry: use at dilution of 1:25-1:100. Detection of ACE2 in paraffin-embedded human brain with #19506 diluted 1:30.
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Antibody Details

Product Details

Reactivity Species
Human
Host Species
Rabbit
Immunogen
Fusion protein corresponding to internal residues of human ACE2.
Product Concentration
Lot Specific
Formulation
PBS, pH 7.3, 50% glycerol, 0.05% sodium azide.
State of Matter
Liquid
Product Preparation
Purified by immunoaffinity chromatography
Storage and Handling
This antibody is stable for at least one (1) year at -20°C.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco


Immunohistochemistry: use at dilution of 1:25-1:100. Detection of ACE2 in paraffin-embedded human brain with #19506 diluted 1:30. Detection of ACE2 in paraffin-embedded human thyroid carcinoma with #19506 diluted 1:30. These are recommended dilutions.

Endusers should determine optimal dilutions for their applications.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Polyclonal Antibody specific to Angiotensin Converting Enzyme 2 (ACE2)
Background
Angiotensin-converting enzyme 2 (ACE2) is a zinc containing metalloenzyme located on the surface of endothelial and other cells in the lungs, arteries, heart, kidney, and intestines. The primary function of ACE2 is to offset activity of angiotensin-converting enzyme (ACE). ACE cleaves angiotensin I into the vasoconstrictor angiotensin II. ACE2 in turn cleaves angiotensin II into the vasodilator angiotensin 1–7. In addition, ACE2 is the main entry point into cells for some coronaviruses including HCoV-NL63, SARS-CoV (the coronavirus that causes SARS) and SARSCoV-2 (the coronavirus that causes COVID-19). More precisely, the binding of the spike S1 protein of SARS-CoV and SARS-CoV2 to the enzymatic domain of ACE2 on the surface of cells results in endocytosis and translocation of both the virus and the enzyme into endosomes located within cells.

Antigen Details

Function
Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis (PubMed:27217402). Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II (PubMed:10969042, PubMed:10924499, PubMed:11815627, PubMed:19021774, PubMed:14504186). Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin (PubMed:10969042, PubMed:11815627). Also cleaves other biological peptides, such as apelins (apelin-13, [Pyr1]apelin-13, apelin-17, apelin-36), casomorphins (beta-casomorphin-7, neocasomorphin) and dynorphin A with high efficiency (PubMed:11815627, PubMed:27217402, PubMed:28293165). In addition, ACE2 C-terminus is homologous to collectrin and is responsible for the trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells via direct interaction, regulating its expression on the cell surface and its catalytic activity (PubMed:18424768, PubMed:19185582). {PubMed:10924499, PubMed:10969042, PubMed:11815627, PubMed:14504186, PubMed:18424768, PubMed:19021774, PubMed:19185582, PubMed:27217402}.; [Isoform 2]: Non-functional as a carboxypeptidase. {PubMed:33077916}.; (Microbial infection) Acts as a receptor for human coronaviruses SARS-CoV and SARS-CoV-2, as well as human coronavirus NL63/HCoV-NL63. {PubMed:14647384, PubMed:15452268, PubMed:15791205, PubMed:15897467, PubMed:19901337, PubMed:24227843, PubMed:32142651, PubMed:32155444, PubMed:32221306, PubMed:32225175, PubMed:33000221, PubMed:33082294, PubMed:33432067}.; [Isoform 2]: (Microbial infection) Non-functional as a receptor for human coronavirus SARS-CoV-2. {PubMed:33077916, PubMed:33432184}.
NCBI Gene Bank ID
UniProt.org

References & Citations

IHC
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.