Anti-ATF4 (Phospho-Ser245) Antibody (43053)

Anti-ATF4 (Phospho-Ser245) Antibody (43053)

Product No.: 43053

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Target
ATF4 (Phospho-Ser245)
Product Type
Polyclonal
Isotype
Rabbit
Applications
WB,IHC

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Data

Immunohistochemistry: Detection of ATF4 (phospho-Ser245) in paraffin-embedded human breast carcinoma tissue.
Immunoblotting: Detection of ATF4 (phospho-Ser245) in extracts of HeLa cells untreated or treated with UV.
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Antibody Details

Product Details

Reactivity Species
Human
Host Species
Rabbit
Immunogen
Peptide sequence that includes phosphorylation sites of Serine 245 (N-R-S(p)-L-P) derived from human ATF4 and conjugated to KLH.
Product Concentration
1.0 mg/ml
Formulation
PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
State of Matter
Liquid
Product Preparation
Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide
Storage and Handling
This antibody is stable for at least one (1) year at -20°C.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~45kDa is detected.

Immunohistochemistry: use at dilution of 1:50- 1:100.

These are recommended working dilutions.

End user should determine optimal dilutions for their applications.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Polyclonal Antibody specific to ATF4 (Phospho-Ser245)
Background
ATF4 encodes a transcription factor that was originally identified as a widely expressed mammalian DNA-binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. ATF4 belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Diseases associated with ATF4 include leukoencephalopathy with vanishing white matter and T-cell leukemia.

Antigen Details

Function
Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro (PubMed:10821277). In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type. {PubMed:10821277, PubMed:15916964, PubMed:18397884, PubMed:22304920}.
NCBI Gene Bank ID
UniProt.org
Research Area
Phosphospecific Antibodies

References & Citations

WB,IHC
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.