Anti-AU5 Antibody (18820)
Data
Immunoblotting: Detection of AU5-tagged protein in 200, 100, and 50ng of E. coli lysate containing tagged fusion protein with #18820 diluted1:5,000. - -
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Antibody DetailsProduct DetailsReactivity Species Au5 Host Species Goat Immunogen AU5 (TDFYLK) conjugated to KLH. Product Concentration 100 μg/ml Formulation PBS (PBS), pH 7.2, 0.09% sodium azide. State of Matter Liquid Product Preparation Purified by peptide affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at 2-8°C. Regulatory Status For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at a dilution of 1:1,000-1:20,000
Immunocytochemistry: use at a dilution of 1:100-1:400 ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100- 1:500. Immunoprecipitation: use at 1-4ug/mg lysate. These are recommended dilutions. End users should determine optimal dilutions for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Antibody was affinity-purified on the peptide. Background AU5 is an epitope tag often constructed on the N- or C- terminus of a protein to allow for detection and analysis of the protein. The AU5 sequence represents the amino acid sequence TDFYLK. Antigen DetailsResearch Area Epitope Tags References & CitationsTechnical Protocols ICC   |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
