Anti-Beta-O-Linked N-Acetylgalactosamine Mouse Monoclonal Antibody (56604)
Anti-Beta-O-Linked N-Acetylgalactosamine Mouse Monoclonal Antibody (56604)
Product No.: 56604
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Clone 9B9 Target O-GalNAC Formats AvailableView All Product Type Monoclonal Alternate Names GalNAC, O-GalNAC, Beta-O-Linked N-Acetylgalactosamine, O-Linked N-Acetylgalactosamine, N-Acetylgalactosamine, 2-(Acetylamino)-2-deoxy-D-galactose, 2-Acetamido-2-deoxy-D-galactose, N-Acetylchondrosamine, 2-Acetamido-2-deoxy-D-galactopyranose, N-Acetyl-D-galactosamine, alpha-N-Acetylgalactosamine, N-Acetylgalactosamine-modified protein Isotype Mouse IgM Applications ELISA , ICC , IF , IHC , WB |
Data
Western Blot analysis of Glycoconjugates showing detection of 67 kDa O-GalNAC protein using Mouse Anti-O-GalNAC Monoclonal Antibody, Clone 9B9 . Lane 1: Molecular Weight Ladder (MW). Lane 2: GlcNAc-BSA. Lane 3: GalNAc-BSA. Lane 4: Galactose-BSA. Lane 5: Glucose-BSA. Load: 2.0 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-O-GalNAC Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GalNAC Monoclonal Antibody, Clone 9B9 . Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GalNAC Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) O-GalNAc Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
Immunohistochemistry analysis using Mouse Anti-O-GalNAc Monoclonal Antibody, Clone 9B9 . Tissue: colon. Species: Mouse. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-O-GalNAc Monoclonal Antibody at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) O-GalNAc Antibody (D) Composite. - -
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Antibody DetailsProduct DetailsReactivity Species Species Independent Host Species Mouse Immunogen Synthetic Beta-O-Linked N-Acetylglucosamine conjugated to Keyhole Limpet Hemocyanin (KLH). Product Concentration 1 mg/mL Formulation PBS pH 7.4, 50% glycerol, 0.09% Sodium azide State of Matter Liquid Product Preparation PEG Purified Storage and Handling This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles. Regulatory Status Research Use Only Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco WB (1:1000); ICC/IF (1:50); ELISA (1:1000); IHC (1:25); Optimal dilutions for assays should be determined by the user. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Specific for Beta-O-Linked N-Acetylgalactosamine and Beta-O-Linked N-Acetylglucosamine modified proteins. Does not cross-react with Glucose or Galactose modified proteins. Antigen DetailsResearch Area Cell Signaling . Glycation . Post-translational Modifications References & CitationsTechnical Protocols ICC IF   |
Formats Available
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Prod No. | Description |
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56604 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
