Anti-Chloride Channel Protein 3 (Clcn3) Antibody (11566)
Anti-Chloride Channel Protein 3 (Clcn3) Antibody (11566)
Product No.: 11566
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Clone S258-5 Target Chloride Channel Protein 3 (Clcn3) Formats AvailableView All Product Type Monoclonal Alternate Names Chloride channel protein 3, ClC-3, Chloride transporter ClC-3 Isotype Mouse IgG1 Applications ICC , IF , IHC , WB |
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Antibody DetailsProduct DetailsReactivity Species Human ⋅ Mouse ⋅ Rat Host Species Mouse Immunogen Synthetic peptide corresponding to aa 98-115 (cytoplasmic N-terminus) of rat Clcn3 (accession no. NP_445815.2). Product Concentration Lot Specific Formulation PBS, pH 7.4; 50% glycerol, 0.09% sodium azide. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -20°C. Avoid repeated freeze-thaw cycles. Regulatory Status For in vitro investigational use only. Not for use in therapeutic or diagnostic procedures. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1-2ug/mL. A band of ~90kDa is detected.
Immunohistochemistry: use at 1-10ug/mL. These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: Rat brain membranes. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This antibody recognizes human, mouse,
and rat Clcn3. It does not cross-react with
Clcn4 or Clcn5. Background Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. Clcn3 mediates the exchange of chloride ions against protons and functions as an antiporter, contributing to the acidification of the endosome and synaptic vesicle lumen. It may play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. Antigen DetailsFunction [Isoform 1]: Strongly outwardly rectifying, electrogenic H(+)/Cl(-)exchanger which mediates the exchange of chloride ions against protons (PubMed:10915634). The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons (By similarity). The presence of conserved gating glutamate residues is typical for family members that function as antiporters (By similarity). {UniProtKB:P51790, PubMed:10915634}.; [Isoform 2]: Strongly outwardly rectifying, electrogenic H(+)/Cl(-)exchanger which mediates the exchange of chloride ions against protons (PubMed:10915634). May play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C (PubMed:8155321). It could help neuronal cells to establish short-term memory (PubMed:8155321). {PubMed:10915634, PubMed:8155321}. NCBI Gene Bank ID UniProt.org Research Area Ion Channels References & CitationsTechnical Protocols |
Formats Available
