This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Mouse Anti-CNS Myelin/Oligodendrocytes (Clone AERA8-154 (CE1)) recognizes Myelin and Oligodendrocytes in the CNS. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
The MOSP antigen is present on oligodendrocytes and CNS myelin but not on Schwann cells or peripheral nervous system (PNS) myelin. No specific staining is observed in sections from PNS, retina, liver, skin, or kidney.
Myelin is an electrically-insulating dielectric phospholipid layer that surrounds the axons of many neurons. It is an outgrowth glial cell: Schwann cells supply the myelin for peripheral neurons, whereas oligodendrocytes supply it to those of the central nervous system. Myelin is considered a defining characteristic of the (gnathostome) vertebrates, but it has also arisen by parallel evolution in some invertebrates. Myelin made by different cell types varies in chemical composition and configuration, but performs the same insulating function. Myelinated axons are white in appearance. Myelin is composed of about 80% lipid fat and about 20% protein. Some of the proteins that make up myelin are Myelin basic protein (MBP), Myelin oligodendrocyte glycoprotein (MOG), and Proteolipid protein (PLP).
Neuroscience Cell Markers
References & Citations
1. Dyer, C. A. et al. (1991) J. of Neuroscience 28:607
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