Anti-Eastern Equine Encephalitis Virus (Clone: EEEV-138) – Purified No Carrier Protein

Sequenced from human survivors of natural EEEV infection.

This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Recombinant antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one year. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≥ -70°C. Avoid Repeated Freeze Thaw Cycles.

The pan-alphavirus clone EEEV-138 binds to EEEV, VEEV, WEEV, CHIKV, and MAYV antigens (VLPs and/or recombinant E1).

Standard Overnight on Blue Ice.

EEEV-138 is a pan-alphavirus antibody reactive against the E1 glycoprotein of alphavirus'.

Eastern Equine Encephalitis virus (EEEV), one of the most virulent viruses endemic to North America, is a rare mosquito-borne encephalitic alphavirus in the Togaviridae family. Infection leads to a 30% to 75% mortality rate, and up to 90% of survivors develop ongoing neurologic problems^{1, 2}. On average, seven human cases are confirmed yearly in the United States. EEEV is of particular concern because of its potential aerosol spread and lack of available treatments. EEEV prevalence in mosquitoes that feed on humans has recently increased.

1. Centers for Disease Control and Prevention, National Center for Emerging and Zoonotic Infectious Diseases (NCEZID), Division of Vector-Borne Diseases (DVBD), Link Text
2. Williamson LE, Gilliland T Jr, Yadav PK, et al. Cell. 183(7):1884-1900.e23. 2020.
3. Kim AS, Austin SK, Gardner CL, et al. Nat Microbiol. 4(1):187-197. 2019.
4. Zhang R, Hryc CF, Cong Y, et al. EMBO J. 30(18):3854-3863. 2011.
5. Voss JE, Vaney MC, Duquerroy S, et al. Nature. 468(7324):709-12. 2010.
6. Li L, Jose J, Xiang Y, et al. Nature. 468(7324):705-8. 2010.