Anti-Eastern Equine Encephalitis Virus (Clone: EEEV-129) – Purified No Carrier Protein
Anti-Eastern Equine Encephalitis Virus (Clone: EEEV-129) – Purified No Carrier Protein
Product No.: LT570
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Product No.LT570 Clone EEEV-129 Target Eastern Equine Encephalitis Virus Product Type Recombinant Monoclonal Antibody Alternate Names EEEV, Triple E Isotype Human IgG1 Applications ELISA , N |
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Antibody DetailsProduct DetailsReactive Species Eastern Equine Encephalitis ⋅ Virus Expression Host HEK-293 Cells Immunogen A panel of human monoclonal antibodies (mAb), including EEEV-129, was isolated and sequenced from the B cells of a survivor of natural EEEV infection 2 Product Concentration ≥1.0 mg/ml Purity ≥90% monomer by analytical SEC and SDS-Page Formulation This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Recombinant antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one year. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≥ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Standard Overnight on Blue Ice. Additional Applications Reported In Literature ? ELISA N: Antibody EEEV-129 was shown to efficiently neutralize Sindbis virus (SINV)/EEEV, a chimeric virus containing nonstructural proteins of SINV and structural proteins of EEEV strain FL93-939. EEEV-129 exhibited potent neutralizing capacity against SINV/EEEV and pathogenic EEEV strain FL93-939. NOTE: EEEV-129 neutralization was also tested against escape mutant viruses and displays a >10-fold reduction in neutralization potency to SINV/EEEV (M68T). Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone EEEV-129 activity is directed against the B domain of the E2 glycoprotein. Furthermore, human monoclonal antibody EEEV-129 binds strongly to both SINV/EEEV particles and EEEV E2 glycoprotein. The Fab form of EEEV-129 also neutralizes SINV-EEEV efficiently but with reduced potency, suggesting bivalent or tetravalent interactions as an IgG may contribute to optimal neutralization of SINV/EEEV. Competition-binding studies utilizing biolayer interferometry shows that EEEV-129 competes with domain B-specific murine anti-EEEV mAbs. Background Eastern Equine Encephalitis virus (EEEV), one of the most virulent viruses endemic to North America, is a rare mosquito-borne encephalitic alphavirus in the Togaviridae family. Infection leads to a 30% to 75% mortality rate, and up to 90% of survivors develop ongoing neurologic problems1, 2. On average, seven human cases are confirmed yearly in the United States. EEEV is of particular concern because of its potential aerosol spread and lack of available treatments. EEEV prevalence in mosquitoes that feed on humans has recently increased.
Research Area Category B Pathogens . Eastern Equine Encephalitis . Infectious Disease . Viral . IVD Raw Material References & Citations1. Centers for Disease Control and Prevention, National Center for Emerging and Zoonotic Infectious Diseases (NCEZID), Division of Vector-Borne Diseases (DVBD), Link Text
2. Williamson LE, Gilliland T Jr, Yadav PK, et al. Cell. 183(7):1884-1900.e23. 2020. Technical ProtocolsCertificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.