Anti-Ezrin (Phospho-Tyr353) Antibody (43063)
Anti-Ezrin (Phospho-Tyr353) Antibody (43063)
Product No.: 43063
Data
Immunohistochemistry: Detection of Ezrin (phospho-Tyr353) in paraffin-embedded human breast carcinoma tissue.
Immunoblotting: Detection of Ezrin (phospho-Tyr353) in extracts of
293 cells untreated or treated with EGF. - -
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Antibody DetailsProduct DetailsReactivity Species Human Host Species Rabbit Immunogen Peptide sequence that includes phosphorylation site of tyrosine 353 (Q-D-Y(p)-E-E) derived from human Ezrin and conjugated to KLH. Product Concentration 1.0 mg/ml Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. State of Matter Liquid Product Preparation Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide Storage and Handling This antibody is stable for at least one (1) year at -20°C. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~81kDa is detected.
Immunohistochemistry: use at dilution of 1:50- 1:100. These are recommended working dilutions. End user should determine optimal dilutions for their application. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rabbit Polyclonal Antibody specific to Ezrin (Phospho-Tyr353) Background Ezrin (also known as cytovillin or villin-2) is encoded by the EZR gene. The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. It plays a key role in cell surface structure adhesion, migration, and organization. The N-terminal FERM domain strongly binds sodium-hydrogen exchanger regulatory factor (NHERF) proteins (involving long-range allostery). The C-terminal ERM domain binds to actin, phosphatidylinositol(4,5)bis-phosphate (PIP2) and membrane proteins like CD44 and ICAM-2. Antigen DetailsFunction Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis. {PubMed:17881735, PubMed:18270268, PubMed:19111582}. NCBI Gene Bank ID UniProt.org Research Area Phosphospecific Antibodies References & CitationsTechnical Protocols  |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
