Anti-Human Alpha Defensin-5 (HD5) – Purified in vivo PLATINUM™ Functional Grade

Clone 8C8 is a monoclonal antibody generated from mouse and is specific for human alpha defensin-5 (HD5), a peptide highly expressed in Paneth cells of the small intestine. Its validated applications are immunohistochemistry and western blotting, typically on human tissue samples—standard use cases involve detection of HD5 in colon or small intestinal tissues.

There is no evidence in the provided search results that clone 8C8 is used in in vivo mouse studies. Available information indicates that its target is human HD5, and the antibody is derived from mouse, but there is no documentation of it being administered to live mice or used to modify mouse physiology or immune responses in vivo. The antibody is characterized by its suitability for in vitro techniques, specifically western blot and immunohistochemistry.

If you are asking whether clone 8C8 has been injected into mice for depletion studies, for tracking cells, or for functional studies, the search results do not support such uses. Instead, it appears this antibody is primarily a research tool for detection of human HD5 in human tissues by immunohistochemical or biochemical analysis.

If you meant a different clone (as other antibodies, like anti-CD8? clones such as 53-6.7, are frequently used in vivo in mice), please clarify. Based on current evidence, clone 8C8 is not conventionally used in in vivo mouse studies as per the available literature and product descriptions.

Storage Temperature for Sterile Packaged Clone 8C8

The search results do not directly specify the correct storage temperature for a sterile packaged clone 8C8 (which appears to be a monoclonal antibody). However, general best practices for storage of sterile reagents—especially those used for research, diagnostics, or therapeutics—can be inferred from related scientific literature and regulatory guidelines.

General Guidelines for Sterile Reagents

• Sterile Biological Reagents: Most antibodies and proteins are optimally stored at 2–8°C short-term, or at –20°C or –80°C for long-term preservation, unless specific instructions indicate otherwise. Lyophilized (freeze-dried) products can often be stored at room temperature before reconstitution.
• Sterile Medical Devices: The environment for sterile packaged items should have maximum room temperature of 25°C (77°F), be dry, clean, protected from light and dust, and free from unsterile objects. This applies to sterile medical devices, which may share some similarities in packaging with biological reagents.
• Compounded Sterile Preparations (CSPs): Controlled room temperature (20–25°C), refrigerated (2–8°C), or frozen storage may be used depending on the product and its shelf life, with strict adherence to manufacturer’s recommendations.

Recommendations Specific to 8C8

Since clone 8C8 is a monoclonal antibody (as referenced in context of ab62757 at Abcam), the normal storage recommendation for such reagents is typically 2–8°C until needed. Always check the product datasheet or manufacturer’s instructions for the specific clone 8C8 in your possession, as variations in formulation or packaging can alter optimal storage conditions. If the datasheet is unavailable, store at 2–8°C as a conservative default.

Summary Table

Key Takeaway

For sterile packaged clone 8C8 (antibody), the correct storage temperature is typically 2–8°C in a refrigerator, unless the manufacturer specifies otherwise. Always verify with the product datasheet or contact the supplier for the most accurate and specific storage instructions. If the product is lyophilized (dry), it may be stored at room temperature until reconstituted, after which refrigeration (2–8°C) is required.

The most commonly referenced antibodies and proteins used alongside 8C8 in the literature are other anti-L1 monoclonal antibodies, specifically 39D4, M12B9, M2E9, and M7B6, which all target the vaccinia virus L1 protein. In experimental setups, these antibodies are frequently used for comparative studies of epitope specificity, binding affinity, and neutralization mechanisms.

Additional relevant details:

• 8C8 and 39D4 each recognize unique epitopes on L1, whereas M2E9, M7B6, and M12B9 share a mutual binding site, according to cross-blocking ELISA assays.
• All five anti-L1 antibodies (8C8, 39D4, M12B9, M2E9, M7B6) are capable of binding refolded L1 protein in ELISA and can immunoprecipitate L1 protein from vaccinia virus-infected cells.
• Experimental generation of 8C8 often involves recombinant fusion proteins, such as GST-tagged or His-tagged versions of L1(1-185), for boosting immunization responses.

For studies outside the vaccinia context (if your query refers to another field or protein), please specify, as the use of “8C8” may have alternative meanings in different research areas. However, in the context of vaccinia virus research, the above antibodies are the most relevant and commonly co-used proteins with 8C8.

Based on the available search results, clone 8C8 appears to be referenced in scientific literature primarily as an antibody clone, specifically the Defensin alpha 5 Antibody (8C8).

Primary Applications and Uses

Clone 8C8 has been documented for use in multiple research applications across various experimental techniques. The antibody demonstrates versatility in laboratory settings, with reported applications including:

Immunological Techniques: The clone has been utilized in flow cytometry experiments, where it shows effectiveness at a concentration of 1 ?g per million cells when used for intracellular staining of MCF-7 cells. In immunohistochemistry applications, it demonstrates moderate nuclear and faint cytoplasmic staining in human skeletal muscle myocytes.

Analytical Methods: Clone 8C8 has been employed in various analytical techniques including dot blot, ELISA, immunocytochemistry/immunofluorescence (at dilutions of 1:200-1:1000), and western blot (at 1:1000 dilution).

Specialized Research Applications

In cardiovascular medicine research, clone 8C8 has been mentioned alongside other antibody clones in studies involving C-reactive protein (CRP) analysis, where it appears to be used in conjunction with other clones for specific protein detection assays.

Methodological Considerations

The literature indicates that when using clone 8C8, researchers should be aware that the observed molecular weight of target proteins may vary from predicted values due to post-translational modifications, cleavages, relative charges, and other experimental factors. This suggests that clone 8C8 requires careful optimization and validation in experimental protocols.

The available evidence suggests that clone 8C8 serves as a reliable research tool across multiple experimental platforms, though the specific findings and outcomes of studies utilizing this clone would require access to the full research papers for more detailed analysis.