Anti-Human APOBEC3G (NT)

PN:A320

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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Rabbit Anti-Human APOBEC3G recognizes an epitope near the N-terminus of Human APOBEC3G. This polyclonal antibody was purified using affinity chromatography.

The Apoliprotein B mRNA-editing, enzyme-catalytic, polypeptide-like (APOBEC) 3 is a multi-isoform member of the cytidine deaminase family of enzymes that act on monomeric nucleoside and nucleotide substrates.¹ Similar to TRIM5α which targets incoming retroviral capsids, APOBEC3 plays a major role in cellular defense against retroviral infection^2,3 as at least two isoforms, APOBEC3G and to a lesser extent APOBEC3F, can be incorporated HIV-1 virions and induce hypermutation in the newly synthesized viral DNA and thus destabilize the viral genome.⁴ This innate mechanism of retroviral resistance is counteracted by the HIV-1 Vif protein by inducing the ubiquitization and degradation of APOBEC3G; a single amino acid substitution (D128K) blocks APOBEC3G depletion without affecting its inhibitory activity.

1. Jarmuz, A. et al. (2002) Genomics 79:285 2. Stremlau, M. et al. (2004) Nature 427:848 3. Bieniasz, P. D. (2004) Nat. Immunol. 5:1109 4. Sheehy, A. M. et al. (2002) Nature 418:646