This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Handling
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day Ambient
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Rabbit Anti-Human Apoptosis signal-regulating kinase (ASK1) recognizes an epitope near the N-terminus of human and mouse ASK1. This polyclonal antibody was purified using affinity chromatography.
Mitogen-activated protein (MAP) kinase cascades are activated in response to various extracellular stimuli, including cytokines, growth factors and environmental stresses. A novel MAP kinase kinase kinase (MAPKKK) was recently identified and designated ASK1 (for apoptosis signal-regulating kinase 1) and MAPKKK5.1-3 ASK1 activated two different subgroups of MAPKK, MKK4 and MKK6, which in turn activated c-Jun N-terminal kinase (JNK) and p38 MAP kinase, respectively. ASK1/MAPKKK5 is activated by TNFR and Fas through the interaction with members of the TRAF family and Fas-associated protein Daxx. Overexpression of ASK1 induced apoptotic cell death, and a catalytically inactive form of ASK1 inhibited TNF-Α-induced apoptosis. ASK1 is expressed in variety of tissues and cell lines.
References & Citations
1. Ichijo, H. et al. (1997) Science 275:90-4.
2. Wang, XS. et al. (1996) J. Biol. Chem. 271:31607-11.
3. Tobiume, K. et al. (1997) M Biochem. Biophys. Res. Commun. 239:905-10.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.