Anti-Human B7-H2 (Clone 136726)

Pricing & Details

Product No.B569
Clone
136726
Protein
B7-H2
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
B7 Homolog 2, B7RP-1, B7h, LICOS, GL50
Isotype
Mouse
IgG2b
Applications
CyTOF®
,
FC
,
N
Prod No.
Size
Price
Avail.
Qty
Add to cart
B569-25 µg
25 µg
$185.00
In stock
Max:
Min: 1
Step: 1
B569-100 µg
100 µg
$389.00
In stock
Max:
Min: 1
Step: 1
B569-500 µg
500 µg
$849.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human B7-H2 (Accession # O75144)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing B7-H2. A suggested method would be to stain cells expressing B7-H2 with 0.25 µl per 1-2.5 x 106 cells in a total staining volume of ≤200 µl followed by PN:M1189.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human B7-H2 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human B7-H2 is 1-4 µg/ml in the presence of 20 ng/mL of anti-human CD3 and 3 μg/mL of rhB7-H2/Fc, using 72 hour PHA-stimulated human T cells.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human B7-H2 (Clone 136726) recognizes an epitope on Human B7-H2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. In direct ELISAs and Western blots, this antibody shows no cross-reactivity with rhB7-1, rhB7-2, rhB7-H1, rhB7-H3, or rmB7-H2.
Background
Human B7-H2, also called B7RP-1, B7h, LICOS, and GL50, is a member of the growing B7 family of immune costimulatory proteins. Other family members include B7-1, B7-2, B7-H1 (PD-L1), PD-L2, and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily, the extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Among the family members, they share about 20 - 25% amino acid identity. Human and mouse B7-H2 share approximately 49% amino acid identity. B7-H2 has been identified as the ligand for ICOS, a member of the CD28 family of costimulatory receptors. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.

Antigen Details

Protein
PubMed

References & Citations

1. Chen, L. et al. (2000) Blood. 96(8):2808-13.
2. Wang, Z. et al. (2018) Int Immunopharmacol. 56:128-134.
Flow Cytometry

Formats Available

Prod No.
Description
B569
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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