Anti-Mouse CD275 – Purified in vivo GOLD™ Functional Grade

Pricing & Details

Product No.C2369
Clone
HK5.3
Protein
B7-H2
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
ICOS-L, B7RP-1, LICOS
Isotype
Rat
IgG2a
Applications
B
,
FC
Prod No.
Size
Price
Avail.
Qty
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C2369-1.0mg
1.0 mg
$105.00
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Max:
Min: 1
Step: 1
C2369-5.0mg
5.0 mg
$355.00
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Max:
Min: 1
Step: 1
C2369-25mg
25 mg
$965.00
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Min: 1
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C2369-50mg
50 mg
$1,595.00
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Min: 1
Step: 1
C2369-100mg
100 mg
$2,300.00
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Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
Transfected Cell Line for Mouse B7H2
Product Concentration
≥ 2.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
Flow Cytometry For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µL volume or 100 µL of whole blood.
Other Applications Reported In Literature ?
Additional reported applications (for the relevant formats) include:
Blocking ligand binding
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse B7-H2 (CD275) (Clone HK5.3) recognizes an epitope on Mouse CD275. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution
splenic B-cells, T-cells, dendritic cells and macrophages
Background
B7-H2, also known as ICOSL and B7RP-1, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. It is a transmembrane glycoprotein with extracellular IgV and IgC domains. B7-H2 is expressed on B cells and macrophages. It binds to ICOS on activated T cells and thus delivers a positive costimulatory signal for optimal T cell function1. ICOS and B7RP-I do not interact with proteins in the CD28-B7 pathway2. The structural features of B7-H2 are crucial for its costimulatory function. B7-H2 constructs lacking either the IgC or IgV domain demonstrates that receptor binding is mediated solely by the IgV domain but requires the IgC domain for maintaining the structural integrity of the protein3.

Antigen Details

Protein
Ligand/Receptor
ICOS
Function
Binds to ICOS. Co-stimulates T cell responses including proliferation and cytokine secretion. Stimulates B cell proliferation and differentiation to plasma cells.
PubMed
NCBI Gene Bank ID
Research Area
Immunology

References & Citations

1. Wiendl, H. et al. (2003) Brain 126:1026
2. Yoshinaga, SK. et al. (1999) Nature 402:827
3. Chattopadhyay, KJ. et al. (2006) Immunol. 177:3920
Flow Cytometry
No results found. Clear filters and try again?

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.