This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Handling
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day Ambient
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Rabbit Anti-Human Bad recognizes an epitope near the C-terminus of human, mouse and rat Bad. This polyclonal antibody was purified using affinity chromatography.
Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death.1,2 Bcl-2 homology 3 (BH3) domain containing pro-apoptotic proteins, such as Bax, Bid, and Bik, form a growing subclass of the Bcl-2 family. Another such protein is the Bcl-2-antagonist of cell death (Bad). Bad regulates apoptosis by forming heterodimers with anti-apoptotic proteins Bcl-2 and Bcl-xL, thereby preventing them from binding with Bax.3 Bad activity is regulated by its phosphorylation; it is inactivated by kinases such as Akt and MAP kinase and thus promotes cell survival,4 whereas JNK-induced phosphorylation promotes the apoptotic role of Bad.5
References & Citations
1. Cory, S. et al. (2003) Oncogene 22:8590-607.
2. Heiser, D. et al. (2004) Exp. Geron. 39:1125-35.
3. Ottilie, S. et al. (1997) J. Biol. Chem. 272:30866-72.
4. Zhou, XM. et al. (2000) J. Biol. Chem. 275:25046-51.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.