Anti-Human Bad

PN:B465

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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Rabbit Anti-Human Bad recognizes an epitope near the C-terminus of human, mouse and rat Bad. This polyclonal antibody was purified using affinity chromatography.

Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death.^1,2 Bcl-2 homology 3 (BH3) domain containing pro-apoptotic proteins, such as Bax, Bid, and Bik, form a growing subclass of the Bcl-2 family. Another such protein is the Bcl-2-antagonist of cell death (Bad). Bad regulates apoptosis by forming heterodimers with anti-apoptotic proteins Bcl-2 and Bcl-xL, thereby preventing them from binding with Bax.³ Bad activity is regulated by its phosphorylation; it is inactivated by kinases such as Akt and MAP kinase and thus promotes cell survival,⁴ whereas JNK-induced phosphorylation promotes the apoptotic role of Bad.⁵

1. Cory, S. et al. (2003) Oncogene 22:8590-607.
2. Heiser, D. et al. (2004) Exp. Geron. 39:1125-35.
3. Ottilie, S. et al. (1997) J. Biol. Chem. 272:30866-72.
4. Zhou, XM. et al. (2000) J. Biol. Chem. 275:25046-51.