Anti-Human BAFF (CT)

Anti-Human BAFF (CT)

Product No.: B359

[product_table name="All Top" skus="B359"]

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Target
BAFF
Product Type
Polyclonal Antibody
Alternate Names
B-Cell Activating Factor, TNFSF13B, TALL-1, BLyS, THANK, zTNF4, BAFF, CD257, TNFSF20, Delta BAFF
Applications
IHC FFPE
,
WB

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Product Size
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Rabbit
Immunogen
Purified Recombinant Human BAFF (>98%)
Product Concentration
0.5 mg/ml
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Handling
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day Ambient
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Anti-Human BAFF recognizes an epitope near the C-terminus of Human, Mouse and Rat BAFF. This polyclonal antibody was purified using affinity chromatography.
Background
B-cell activating factor (BAFF) is the tumor necrosis factor ligand superfamily member 13B (TNFLSF13B), also known as B Lymphocyte Stimulator (BLyS) and TNF- and APOL-related leukocyte expressed ligand (TALL-1) and the Dendritic cell-derived TNF-like molecule (CD257 antigen; cluster of differentiation 257).

BAFF is a glycoprotein which undergoes glycosylation at residue 124. It is expressed as transmembrane protein on various cell types including monocytes, dendritic cells and bone marrow stromal cells. The transmembrane form can be cleaved from the membrane, generating a soluble protein fragment. BAFF is the natural ligand of three unusual tumor necrosis factor receptors named BAFF-R, TACI, and BCMA, all of which have differing binding affinities for it. These receptors are expressed mainly on mature B lymphocytes (TACI is also found on a subset of T-cells and BCMA on plasma cells). TACI binds worst since its affinity is higher for a protein similar to BAFF, called A Proliferation Inducing Ligand (APRIL). BCMA displays an intermediate binding phenotype and will work with either BAFF or APRIL to varying degrees. Signaling trough BAFF-R and BCMA stimulates B lymphocytes to undergo proliferation and to counter apoptosis. All these ligands act as heterotrimers (i.e. three of the same molecule) interacting with heterotrimeric receptors.

BAFF provides critical survival signals to a subset of B cells with intermediate maturation status (T2 B cells) during the immune response. BAFF also plays an important role in the development of lymphoid tissue and enhances the survival of activated memory B cells. Human and mouse BAFF share 86% aa sequence identity.

Antigen Details

PubMed
NCBI Gene Bank ID

References & Citations

1. Moore, P. A. et al. (1999) Science 285:260 2. Schneider, P. et al. (1999) J. Exp. Med. 189:1747 3. Shu, H. B. et al. (1999) J. Leukoc. Biol. 65:680 4. Mukhopadhyay, A. et al. (1999) J. Biol. Chem. 274:15978
IHC FFPE
General Western Blot Protocol
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.