Anti-Human Bcl-Rambo (Intermediate Domain)

PN:B475

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Next Day Ambient

Rabbit Anti-Human Bcl-rambo recognizes an epitope in the intermediate domain of human, mouse and rat Bcl-rambo. This polyclonal antibody was purified using affinity chromatography.

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer.¹ Members of the Bcl-2 family are known to be critical regulators of this process. These proteins are characterized by the presence of several conserved motifs termed Bcl-2 homology (BH) domains.^2,3 A novel, widely expressed member termed Bcl-rambo was recently identified. This protein is localized to mitochondria in mammalian cells and its overexpression induces apoptosis which could be blocked by co-expression of inhibitor of apoptosis proteins (IAPs) such as XIAP, cIAP1, and cIAP2.⁴ Bcl-rambo shows overall homology to the anti-apoptotic members containing BH motifs, but unlike Bcl-2, the C-terminal membrane anchor of Bcl-rambo is preceded by a unique 250 amino acid insertion. This region by itself can induce apoptosis more efficiently than the Bcl-2 homology regions, suggesting that Bcl-rambo may be important other pro-apoptotic pathways.⁴

1. Lockshin, RA. et al. (2000) Cell Death Differ. 7:2-7.
2. Cory, S. et al. (2003) Oncogene 22:8590-607.
3. Heiser, D. et al. (2004) Exp. Geron. 39:1125-35.
4. Kataoka, T. et al. (2001) J. Biol. Chem. 276:19548-54.