This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Handling
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day Ambient
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Rabbit Anti-Human Bcl-rambo recognizes an epitope in the intermediate domain of human, mouse and rat Bcl-rambo. This polyclonal antibody was purified using affinity chromatography.
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer.1 Members of the Bcl-2 family are known to be critical regulators of this process. These proteins are characterized by the presence of several conserved motifs termed Bcl-2 homology (BH) domains.2,3 A novel, widely expressed member termed Bcl-rambo was recently identified. This protein is localized to mitochondria in mammalian cells and its overexpression induces apoptosis which could be blocked by co-expression of inhibitor of apoptosis proteins (IAPs) such as XIAP, cIAP1, and cIAP2.4 Bcl-rambo shows overall homology to the anti-apoptotic members containing BH motifs, but unlike Bcl-2, the C-terminal membrane anchor of Bcl-rambo is preceded by a unique 250 amino acid insertion. This region by itself can induce apoptosis more efficiently than the Bcl-2 homology regions, suggesting that Bcl-rambo may be important other pro-apoptotic pathways.4
References & Citations
1. Lockshin, RA. et al. (2000) Cell Death Differ. 7:2-7.
2. Cory, S. et al. (2003) Oncogene 22:8590-607.
3. Heiser, D. et al. (2004) Exp. Geron. 39:1125-35.
4. Kataoka, T. et al. (2001) J. Biol. Chem. 276:19548-54.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.