Anti-Human Bfl-1 (CT)

PN:B484

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Next Day Ambient

Rabbit Anti-Human Bfl-1 recognizes an epitope near the C-terminus of human and mouse Bfl-1. This polyclonal antibody was purified using affinity chromatography.

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by caspase activation. Proteins that comprise the Bcl-2 family appear to control the activation of these enzymes. One such member is multi-domain antiapoptotic protein Bfl-1, which is overexpressed in stomach and other cancers. Bfl-1 can interact with Bax and suppress apoptosis by inhibiting the release of cytochrome c and caspase-3 activation. It is upregulated in cisplatin-resistant human bladder tumor, suggesting that its expression may be important for cisplatin resistance and inhibition of apoptosis in cancer cells. At least two isoforms of Bfl-1 are known to exist. Presumably due to post-translational modifications, Bfl-1 is often observed at higher molecular weight in SDS-PAGE than its predicted molecular weight would suggest.

1. Lockshin, RA. et al. (2000) Cell Death Differ. 7:2-7.
2. Choi, SS. et al. (1995) Oncogene 11:1693-8.
3. Kim, JK. et al. (2004) Cancer Lett. 212:61-70.
4. Zhang, H. et al. (2000) J. Biol. Chem. 275:11092-9