This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Handling
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day Ambient
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Rabbit Anti-Human Bnip3L recognizes an epitope in the intermediate domain of Human Bnip3L. This polyclonal antibody was purified using affinity chromatography.
Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, Hrk, Nip3, and Bim, form a growing subclass of the Bcl-2 family. A novel BH3 domain containing protein was recently identified and designated Bnip3L, Bnip3α, and Nix (for Nip3-like protein X).1-3 Bnip3L/Bnip3α/Nix is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Overexpression of Bnip3L induces apoptosis.2,3 Bnip3L interacts with and overcomes suppresses by Bcl-2 and Bcl-xL. Bnip3L is localized in mitochondria. The messenger RNA of Bnip3L is ubiquitously expressed in human tissues.1,2 Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic mitochondrial proteins.
References & Citations
1. Matsushima, M. et al. (1998) Genes Chromosomes Cancer 21:230-5
2. Yasuda, M. et al. (1999) Cancer Res 59:533-7
3. Chen, G. et al. (1999) J Biol Chem 274(1):7-10
4. Imazu, T. et al. (1999) Oncogene. 18:4523-9
Products are for research use only. Not for use in diagnostic or therapeutic procedures.