This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Handling
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Applications and Recommended Usage ?
(Quality Tested by Leinco)
Western Blotting: To detect Human CCR5 this polyclonal antibody can be used at a concentration of 1 µg/ml.
Rabbit Anti-Human CCR5 recognizes an epitope near the N-terminus of Human CCR5. This polyclonal antibody was purified using affinity chromatography.
Human immunodeficiency virus (HIV) and related virus require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors. Among them, CCR5 (CC-CKR-5) is a principal coreceptor for macrophage- and dual-tropic HIV-1 strains fusion and entry of human white blood cells. CCR5 is required for the infection by HIV-1, HIV-2, and SIV. The b-chemokines RANTES, MIP-1α and MIP-1β are the ligands for CCR5 and prevent infection by M-tropic HIV-1. CXC5 associates with the surface CD4-gp120 of HIV complex and leads to membrane fusion and virus entry of target cells. The amino-terminal domain and the extracellular loops of CCR5 serve as HIV biding sites. CCR5 messenger RNA is expressed in lymphoid organs and monocytes.
NCBI Gene Bank ID
References & Citations
1. Dimitrov, D. S. (1997) Cell 91:721 2. Littman, D. R. (1998) Cell 93:677 3. Deng, H. et al. (1996) Nature 381:661 4. Dragic, T. et al. (1996) Nature 381:667
Products are for research use only. Not for use in diagnostic or therapeutic procedures.