Anti-Human CD18 (ITGB2) (Clone IB4) – Purified in vivo GOLDTM Functional Grade
Anti-Human CD18 (ITGB2) (Clone IB4) – Purified in vivo GOLDTM Functional Grade
Product No.: C930
Clone IB4 Target CD18 Formats AvailableView All Product Type Hybridoma Monoclonal Antibody Alternate Names Integrin beta-2, integrin beta chain, beta 2, ITGB2 Isotype Mouse IgG2a k Applications FA , FC , IF Microscopy , in vivo |
Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Recommended Isotype Controls Recommended Dilution Buffer Immunogen Human peripheral blood lymphocytes Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. State of Matter Liquid Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Regulatory Status Research Use Only Country of Origin USA Shipping 2-8°C Wet Ice Applications and Recommended Usage? Quality Tested by Leinco FC, IHC, IP Additional Applications Reported In Literature ? IF Microscopy, FA Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity IB4 activity is directed against human CD18 and can also stain rabbit bone marrow leukocytes. Background Integrins are a large family of α/β heterodimeric transmembrane molecules that mediate adhesion, migration, cell survival, and cell differentiation1. The leukocytic cell-adhesion molecule (Leu-CAM) family has three members, LFA-1 (CD18/CD11a), CR3 (CD18/CD11b), and p150,95 (CD18/CD11c), all of which share the β chain CD182.
Murine (m) 1B4 was humanized (h) by the CDR-grafting method to create an anti-CD18 antibody with similar avidity, specificity, and efficacy as the murine antibody3. In rhesus monkeys, h1B4 is less immunogenic than m1B4 and elicits only anti-idiotypic antibodies. Additionally, h1B4 and m1B4 exhibit equivalent staining specificity and intensity in rabbit bone marrow leukocytes. For humanization, human VH and VL FR were chosen based on high identity with their corresponding m1B4 FR3. Once grafted with the m1B4 CDR, the human VH and VL FR were expressed as fusion proteins with the IgG4 and Cκ human constant regions, respectively. The h1B4 monoclonal antibody has been used in multiple studies of adhesion in different model systems4, 5, 6 and to measure the expression of CD18 in B-ALL cell lines7. Antigen Distribution Depending on its heterodimeric configuration, CD18 is expressed in leukocytes, monocytes, macrophages, some lymphocytes, polymorphonuclear lymphocytes (PMNs), natural killer cells, neutrophils, granulocytes, certain lymphocytic tumor cell lines, macrophage foam cells, activated platelets, and splenic red pulp macrophages. Ligand/Receptor Receptor NCBI Gene Bank ID UniProt.org Research Area Cell Adhesion . Cell Biology . Immunology . Signal Transduction Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Common in vivo applications of clone IB4 (Isolectin B4, Griffonia simplicifolia agglutinin) in mice primarily involve selective labeling and manipulation of non-peptidergic nociceptive neurons and endothelial cells, especially in contexts such as pain research and vascular biology. Key in vivo applications include:
Important distinctions:
In summary, IB4 is widely used in vivo in mice to selectively label, trace, and manipulate specific subpopulations of neurons and endothelial cells, especially in pain and neurovascular research. Commonly used antibodies and proteins with IB4 (Isolectin B4) in the literature include markers for neuronal subtypes, ion channels, signaling proteins, and extracellular matrix components. These allow researchers to discriminate between distinct cell populations or elucidate molecular mechanisms in tissues where IB4 is used. Frequently co-used antibodies or proteins:
Contextual usage notes:
Summary table:
These markers represent the most common antibodies or proteins employed with IB4 for immunostaining, classification, and functional experiments in neuroscience and cell biology literature. Clone IB4 in scientific literature broadly refers to two distinct entities: 1) Isolectin B4 (IB4) used for labeling specific neuronal populations, and 2) the anti-CD18 monoclonal antibody, clone IB4 used for immunological studies. Below are the key findings associated with these IB4 citations, with a focus on their scientific uses and novel discoveries. 1. Isolectin B4 (IB4): Key Findings
2. Anti-CD18 Monoclonal Antibody, Clone IB4: Key Findings
3. Major Advances Enabled by IB4 Tools
Summary Table: Key Applications and Findings
Most citations regarding clone IB4 concern the use of Isolectin B4 as a neuroscientific tool and as a cell selection molecule in biotechnology. Recent studies emphasize its power to isolate and manipulate subpopulations of neurons or non-human cells for research in pain, regeneration, and transplantation. Dosing Regimens of Clone IB4 in Mouse Models: Current EvidenceSummary: What Is Known About IB4
Reference Dosing Regimens for Other Monoclonal AntibodiesWhile not directly relevant to IB4, it is worth noting how dosing regimens for other monoclonal antibodies are commonly described in the literature:
ConclusionThere is no published information in the provided search results about the dosing regimen of clone IB4 in mouse models. Researchers interested in using IB4 in vivo should consult primary research articles, product sheets, or directly contact the supplier for guidance on in vivo use, potential toxicity, and optimal dosing. Any in vivo use of IB4 should be carefully validated empirically, with close monitoring of animal welfare and experimental outcomes, as dosing regimens for monoclonal antibodies can vary significantly depending on the target, antibody isotype, and experimental model. References & Citations1. Luo BH, Carman CV, Springer TA. Annu Rev Immunol. 25:619-647. 2007.
2. Sanchez-Madrid F, Nagy JA, Robbins E, et al. J Exp Med. 158(6):1785-1803. 1983. 3. Singer II, Kawka DW, DeMartino JA, et al. J Immunol. 150(7):2844-2857. 1993. 4. Hanlon WA, Stolk J, Davies P, et al. J Leukoc Biol. 50(1):43-48. 1991. 5. Seth R, Salcedo R, Patarroyo M, et al. FEBS Lett. 282(1):193-196. 1991. 6. McDonald RJ, St George JA, Pan LC, et al. Inflammation. 17(2):145-151. 1993. 7. Velázquez-Avila M, Balandrán JC, Ramírez-Ramírez D, et al. Leukemia. 33(6):1337-1348. 2019. Technical ProtocolsCertificate of Analysis |
Formats Available
Prod No. | Description |
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C932 | |
C933 | |
C934 | |
C935 | |
C939 | |
C940 | |
C941 | |
C942 | |
C930 |
