Anti-Human CD20 (Obinutuzumab) [Clone GA101] — Biotin

Anti-Human CD20 (Obinutuzumab) [Clone GA101] — Biotin

Product No.: LT914

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Product No.LT914
Clone
GA101
Target
CD20
Product Type
Biosimilar Recombinant Human Monoclonal Antibody
Alternate Names
Obinutuzumab, CD20, MS4A1
Isotype
Human IgG1κ
Applications
ELISA
,
FC

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Human
Expression Host
HEK-293 Cells
Immunogen
Human lymphoblastoid cell line SB.
Product Concentration
0.5 mg/ml
Formulation
This Biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
State of Matter
Liquid
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
Regulatory Status
Research Use Only (RUO). Non-Therapeutic.
Country of Origin
USA
Shipping
2-8°C Wet Ice
Applications and Recommended Usage?
Quality Tested by Leinco
FC,
ELISA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
This non-therapeutic biosimilar antibody uses the same variable region sequence as the therapeutic antibody Obinutuzumab. This product is for research use only. Obinutuzumab (GA101) activity is directed against human CD20.
Background
CD20 is a nonglycosylated 33-37 kDa phosphoprotein member of the MS4A family which is widely expressed on normal B cell surfaces during all stages of development as well as by most B cell malignancies1,2. The biological role of CD20 remains poorly understood; however, it is thought to be involved in calcium ion influx. CD20 has no natural ligand and is not immediately internalized upon antibody binding. Thus, mAbs directed against CD20 depend on the recruitment of a host response. Anti-CD20 mAbs bind to the 44 amino acid extracellular portion.

Obinutuzumab (GA101) is a new generation, type II, anti-CD20 antibody2. Obinutuzumab was humanized by grafting the complementarity-determining sequences of murine IgG1-κ antibody B-Ly1 onto human VH and VL acceptor frameworks3. The Fc segment was glycoengineered to attach bisected, complex, nonfucosylated oligosaccharides to asparagine 297, leading to increased affinity to FcgRIII.

Obinutuzumab causes homotypic adhesion4,5,6, induces direct cell death via largely caspase-independent mechanisms4,6,7,8,9, does not localize into lipid rafts4,10,11, displays half-maximal CD20 binding at saturating conditions7, and displays minimal complement dependent cytotoxicity7.

Compared to rituximab, obinutuzumab recognizes a distinct but overlapping CD20 epitope, in a different orientation that results in increased pro-apoptotic potential12,13,14. A modified elbow-hinge residue, characterized by a leucine to valine mutation at Kabat position 11, is key to superior phosphatidylserine exposure and cell death relative to rituximab3.
Antigen Distribution
CD20 is a general B cell marker expressed by the majority of normal B cells in all stages of their development as well as by most B cell malignancies.
Ligand/Receptor
Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
PubMed
NCBI Gene Bank ID
UniProt.org
Research Area
Biosimilars
.
Cancer
.
Immunology
.
Oncology

Leinco Antibody Advisor

Powered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments.

Research-grade Obinutuzumab biosimilars are commonly used as calibration standards or reference controls in pharmacokinetic (PK) bridging ELISA assays to measure drug concentration in serum samples. These biosimilars enable the construction of standard curves and quality control samples critical for the quantitative assessment of both the biosimilar and reference antibody concentrations in biological matrices.

Essential context and supporting details:

  • Single Analytical Standard: Best industry practice is to develop a single PK assay using one analytical standard—often the research-grade biosimilar Obinutuzumab—for quantifying both the biosimilar and its reference product in serum. This approach reduces variability and simplifies data interpretation, as it ensures that both products are measured with the same calibration curve under identical assay conditions.
  • Assay Calibration: The biosimilar is reconstituted at known concentrations in serum to create a standard curve that spans the expected range of drug concentrations. These standards are included in each ELISA plate run. By comparing the sample responses to the standard curve, absolute concentrations of Obinutuzumab in serum samples can be determined.
  • Reference Controls and QC Samples: Besides standards, biosimilars are also used as reference controls or QC samples at different concentrations throughout the assay to monitor assay accuracy, precision, and robustness. QC samples may include both biosimilar-derived material and material from the licensed reference product to confirm equivalence in quantification.
  • Bioanalytical Equivalence: During assay validation, both the biosimilar and the reference product are spiked into human serum at defined levels and quantified using the biosimilar standard curve. The results are statistically analyzed to confirm that the assay quantifies both products equivalently (typically within predefined limits such as a 90% confidence interval within 0.8–1.25).
  • Kit Implementation: Commercial ELISA kits (for example, from Abbexa, MyBioSource, or Bio-Rad) provide lyophilized or liquid biosimilar standards specifically for constructing the calibration curves, with defined sensitivity and detection ranges suitable for quantifying Obinutuzumab in serum or plasma.
  • Research Use Only: These biosimilar standards and controls are strictly for research or non-clinical PK bioequivalence studies and not for diagnostic or therapeutic use.

Key steps in using biosimilars as standards in PK bridging ELISA:

  • Prepare a series of calibrator standards by diluting the research-grade Obinutuzumab biosimilar in blank serum.
  • Add calibrators, controls, and serum samples to ELISA plates, and develop the assay per validated protocols.
  • Construct the standard curve from calibrator responses and interpolate concentrations for unknown samples.
  • Include reference controls to ensure ongoing assay validity and support regulatory requirements for assay precision and accuracy.

This approach underpins the regulatory and scientific rigor necessary for biosimilar PK comparability studies, ensuring both the test (biosimilar) and reference Obinutuzumab can be reliably measured and compared in clinical pharmacokinetic analyses.

Standard flow cytometry protocols involving a conjugated Obinutuzumab biosimilar (e.g., PE- or APC-labeled) to validate CD20 expression levels or binding capacity typically follow these foundational steps:

  • Cell Preparation: Target cells (e.g., primary B cells, lymphoma cell lines) are washed and suspended in staining buffer (often PBS with 1–2% BSA or FBS).
  • Incubation with Conjugated Antibody: The cells are incubated with the fluorochrome-conjugated Obinutuzumab biosimilar under controlled conditions, usually at 4°C in the dark for 20–30 minutes to allow specific binding to CD20.
  • Washing: Cells are washed to remove unbound antibody, reducing background fluorescence.
  • Flow Cytometric Analysis: Cells are analyzed for fluorescence intensity using the appropriate laser/filter setup for the conjugate (e.g., PE or APC), quantifying CD20 on the cell surface by median fluorescence intensity (MFI).

Validation of CD20 Expression & Binding

  • Specific staining is demonstrated by a significant signal shift in the target cell population when stained with the conjugated antibody compared to unstained or isotype-stained controls.
  • Controls commonly include:
    • Unstained cells.
    • Cells stained with fluorochrome-conjugated isotype control antibody.
    • Cells with competitive binding using excess unlabeled Obinutuzumab biosimilar, demonstrating specificity by reduction of fluorescence signal.
  • Quantification uses MFI measurements normalized by instrument-calibrated fluorescence beads (such as the EuroFlow standardization technique) to account for technical variability.

Protocols for Binding Capacity

  • To assess binding capacity (i.e., how well the conjugated antibody recognizes CD20), serial dilutions of the conjugated Obinutuzumab biosimilar are used to generate a binding curve, enabling calculation of binding parameters such as dissociation constant (( K_D )) and maximum binding (Bmax).
  • These data are usually analyzed by fitting the MFI vs. antibody concentration curve using flow cytometry data analysis software.
  • Some studies use F(ab′)₂ fragments of Obinutuzumab for binding assessments and compare with whole antibody labeling.
StepPurposeNotes
Cell PreparationEnsures accurate surface antigenWash with PBS/BSA; keep on ice to preserve epitopes
StainingSpecific CD20 labelingIncubate with PE/APC–Obinutuzumab biosimilar; control for non-specific binding
WashingReduces backgroundEssential for reliable MFI measurement
ControlsValidates specificityInclude isotype, competitive inhibition, and unstained controls
Data AcquisitionQuantifies CD20Analyze MFI, normalize with beads, report longitudinal/baseline CD20 expression
Data AnalysisAffinity/Expression quantitationCurve fitting for binding; EuroFlow normalization for longitudinal studies

Key Insights

  • Obinutuzumab biosimilar conjugates are suitable for quantifying CD20 expression and assessing binding capacity via flow cytometry, with validation steps necessary for specificity and reproducibility.
  • The EuroFlow bead-based normalization improves comparability and controls for variation in instrument response when quantifying CD20 via MFI.
  • Binding is confirmed with a strong fluorescence shift on CD20^+ cells and minimal binding to CD20^– controls or when using competitive inhibition.

References to Specific Protocol Components

  • Direct antibody conjugate protocols for CD20 assessment are detailed in studies using both Western blot validation and flow cytometric quantitation.
  • Flow cytometry protocols commonly require antibody titration and controls for robust assessment of expression and binding; normalization procedures further refine measurement accuracy.

If you require a detailed protocol, including buffer compositions, antibody concentrations, or gating strategies, please specify your application (e.g., clinical validation, biosimilar characterization, or preclinical studies), as protocols can be adapted based on sample type and regulatory requirements.

Biopharma companies typically perform a comprehensive suite of analytical assays to confirm the structural and functional similarity of a proposed biosimilar to the originator drug, focusing on critical quality attributes (CQAs) relevant to safety, efficacy, and clinical outcomes. Assays cover physicochemical, structural, and biological properties, and Leinco biosimilars are commonly employed as well-characterized reference standards or controls in these studies when available.

Key Analytical Assays for Biosimilarity:

  • Structural Assays:

    • Primary structure analysis: Peptide mapping, mass spectrometry, amino acid sequencing to confirm the biosimilar’s amino acid sequence matches the originator.
    • Secondary and tertiary structure: Circular dichroism, FTIR spectroscopy, NMR, and X-ray crystallography to probe folding and conformation.
    • Higher-order structure: Differential scanning calorimetry and other orthogonal methods to detect subtle conformational differences.
    • Post-translational modifications: Glycosylation profiling, oxidation, deamidation, and sialylation studies using LC-MS and HPLC.
    • Aggregate and impurity analysis: SEC, AUC, SDS-PAGE to compare size variants, aggregates, precursors, fragments, and process-related impurities.
  • Functional Assays:

    • Biological potency assays: Assess actual activity compared to the originator, e.g., cell-based assays such as proliferation, cytotoxicity, or reporter gene assays.
    • Binding assays: ELISA, SPR (Biacore), Fc receptor binding (for antibodies), target or antigen binding by the biosimilar vs originator.
    • Enzyme kinetics: For enzyme biologics, compare rate and mechanism of substrate conversion.
    • Immunological assays: Assess immune effector functions (e.g., ADCC, CDC for antibodies) for functional equivalence.
  • Statistical Comparisons:

    • Equivalence tests and K sigma comparisons to determine whether measured attributes fall within defined similarity ranges.

Role of Leinco Biosimilars in Analytical Similarity Studies:
Leinco provides high-quality biosimilar reagents, such as monoclonal antibodies and recombinant proteins, which can serve two main functions:

  • Reference Standards: Leinco biosimilars may act as side-by-side comparators in structural and functional assays, particularly when originator standards are limited or very costly.
  • Controls in Assays: They offer validated controls for assay qualification and method development, supporting robust and reproducible side-by-side testing.

When a biopharma company is developing a biosimilar, using Leinco’s characterized biosimilar reagents can strengthen assay confidence and reliability, enabling more accurate attribution of similarity or difference to the tested molecule rather than assay variability. These reagents are especially useful in orthogonal testing strategies, as recommended by regulatory guidelines.

Additional Considerations:

  • Complementary orthogonal methods (using techniques that measure the same attribute differently) are often employed to improve sensitivity and reduce the risk of missing subtle differences.
  • Stability and stress-testing assays (e.g., accelerated stability studies) investigate whether product performance remains equivalent over time and under varying storage conditions.

In summary, biosimilar analytical characterization integrates multiple structural and functional assays, with products like Leinco biosimilars used as high-quality reference materials to calibrate and validate these studies, thus helping biopharma companies confidently demonstrate biosimilarity to regulators.

References & Citations

1. Middleton O, Wheadon H, Michie AM. Classical Complement Pathway. In MJH Ratcliffe (Ed.), Reference Module in Biomedical Sciences Encyclopedia of Immunobiology Volume 2 (pp. 318-324). Elsevier. 2016.
2. Freeman CL, Sehn LH. Br J Haematol. 182(1):29-45. 2018.
3. Mössner E, Brünker P, Moser S, et al. Blood. 115(22):4393-4402. 2010.
4. Chan HT, Hughes D, French RR, et al. Cancer Res. 63(17):5480-5489. 2003.
5. Ivanov A, Beers SA, Walshe CA, et al. J Clin Invest. 119(8):2143-2159. 2009.
6. Alduaij W, Ivanov A, Honeychurch J, et al. Blood. 117(17):4519-4529. 2011.
7. Herter S, Herting F, Mundigl O, et al. Mol Cancer Ther. 12(10):2031-2042. 2013.
8. Honeychurch J, Alduaij W, Azizyan M, et al. Blood. 119(15):3523-3533. 2012.
9. Golay J, Zaffaroni L, Vaccari T, et al. Blood. 95(12):3900-3908. 2000.
10. Cragg MS, Morgan SM, Chan HT, et al. Blood. 101(3):1045-1052. 2003.
11. Cragg MS, Glennie MJ. Blood. 103(7):2738-2743. 2004.
12. Niederfellner G, Lammens A, Mundigl O, et al. Blood. 118(2):358-367. 2011.
13. Klein C, Lammens A, Schäfer W, et al. MAbs. 5(1):22-33. 2013.
14. Könitzer JD, Sieron A, Wacker A, Enenkel B. PLoS One. 10(12):e0145633. 2015.
15. Terszowski G, Klein C, Stern M. J Immunol. 192(12):5618-5624. 2014.
16. Bologna L, Gotti E, Manganini M, et al. J Immunol. 186(6):3762-3769. 2011.
17. Ysebaert L, Laprévotte E, Klein C, Quillet-Mary A. Blood Cancer J. 5(11):e367. 2015.
18. Cartron G, Hourcade-Potelleret F, Morschhauser F, et al. Haematologica. 101(2):226-234. 2016.
Indirect Elisa Protocol
Flow Cytometry

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Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.