Anti-Human CD20 (Obinutuzumab) [Clone GA101] — FITC

Anti-Human CD20 (Obinutuzumab) [Clone GA101] — FITC

Product No.: LT913

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Product No.LT913
Clone
GA101
Target
CD20
Product Type
Biosimilar Recombinant Human Monoclonal Antibody
Alternate Names
Obinutuzumab, CD20, MS4A1
Isotype
Human IgG1κ
Applications
ELISA
,
FC

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Human
Expression Host
HEK-293 Cells
Immunogen
Human lymphoblastoid cell line SB.
Product Concentration
0.2 mg/ml
Formulation
This Fluorescein (FITC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
State of Matter
Liquid
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
Regulatory Status
Research Use Only (RUO). Non-Therapeutic.
Country of Origin
USA
Shipping
2-8°C Wet Ice
Excitation Laser
Blue Laser (490 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
FC,
ELISA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
This non-therapeutic biosimilar antibody uses the same variable region sequence as the therapeutic antibody Obinutuzumab. This product is for research use only. Obinutuzumab (GA101) activity is directed against human CD20.
Background
CD20 is a nonglycosylated 33-37 kDa phosphoprotein member of the MS4A family which is widely expressed on normal B cell surfaces during all stages of development as well as by most B cell malignancies1,2. The biological role of CD20 remains poorly understood; however, it is thought to be involved in calcium ion influx. CD20 has no natural ligand and is not immediately internalized upon antibody binding. Thus, mAbs directed against CD20 depend on the recruitment of a host response. Anti-CD20 mAbs bind to the 44 amino acid extracellular portion.

Obinutuzumab (GA101) is a new generation, type II, anti-CD20 antibody2. Obinutuzumab was humanized by grafting the complementarity-determining sequences of murine IgG1-κ antibody B-Ly1 onto human VH and VL acceptor frameworks3. The Fc segment was glycoengineered to attach bisected, complex, nonfucosylated oligosaccharides to asparagine 297, leading to increased affinity to FcgRIII.

Obinutuzumab causes homotypic adhesion4,5,6, induces direct cell death via largely caspase-independent mechanisms4,6,7,8,9, does not localize into lipid rafts4,10,11, displays half-maximal CD20 binding at saturating conditions7, and displays minimal complement dependent cytotoxicity7.

Compared to rituximab, obinutuzumab recognizes a distinct but overlapping CD20 epitope, in a different orientation that results in increased pro-apoptotic potential12,13,14. A modified elbow-hinge residue, characterized by a leucine to valine mutation at Kabat position 11, is key to superior phosphatidylserine exposure and cell death relative to rituximab3.
Antigen Distribution
CD20 is a general B cell marker expressed by the majority of normal B cells in all stages of their development as well as by most B cell malignancies.
Ligand/Receptor
Src family tyrosine kinases, MHC class I, II, CD53, CD81, CD82
PubMed
NCBI Gene Bank ID
UniProt.org
Research Area
Biosimilars
.
Cancer
.
Immunology
.
Oncology

Leinco Antibody Advisor

Powered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments.

Research-grade Obinutuzumab biosimilars are primarily used as calibration standards or reference controls in pharmacokinetic (PK) bridging ELISA by serving as the quantifiable standard against which serum drug concentrations are measured. Calibration standards are prepared using the biosimilar and are serially diluted in serum matrix to produce a standard curve, which allows for the interpolation of unknown sample concentrations.

Context and Key Steps:

  • Single Analytical Standard Usage: Current best practices in biosimilar PK bioanalysis recommend using a single, well-characterized biosimilar (or reference biologic) as the analytical standard for quantifying both the biosimilar and reference product concentrations in serum samples with the same ELISA method. This approach reduces variability inherent in running parallel assays and enhances comparability, which is essential for demonstrating bioequivalence.
  • Standard Curve Preparation: The biosimilar is reconstituted and serially diluted in a blank serum matrix to generate a calibration curve that covers the expected concentration range in study samples. The standard concentrations commonly span nanogram to low microgram per milliliter ranges, for example, 50 to 12,800 ng/mL.
  • ELISA Quantification: On each assay plate, calibration standards (the biosimilar at known concentrations), QC samples (biosimilar spiked at low, medium, high concentrations), and study serum samples are analyzed together. The assay (typically a sandwich or competitive ELISA) uses anti-obinutuzumab antibodies as capture and detection reagents. The resulting optical densities from the standards establish the reference curve, and the drug concentrations in unknown serum samples are quantified by interpolation from this curve.
  • Qualification and Validation: Assay development includes demonstrating that the biosimilar standard yields comparable quantification for both the biosimilar and the reference product. Precision, accuracy, and parallelism are assessed and, where suitable, the biosimilar is validated as the single standard for broader bridging studies.
  • Regulatory and Quality Considerations: Use of a research-grade biosimilar as a calibration standard in PK bridging assays is intended solely for research and non-clinical use. For regulated studies, the performance of the biosimilar standard must be thoroughly validated to ensure equivalence to the reference product in the context of the specific assay.

Additional Relevant Details:

  • Calibration standards are typically lyophilized for storage stability and reconstituted just prior to assay use.
  • Careful validation is required to ensure that the biosimilar and reference product are interchangeable within the assay and that the single-standard method is suitable for PK studies supporting biosimilar bioequivalence claims.

Summary Table: Role of Obinutuzumab Biosimilar in PK Bridging ELISA

StepDescriptionSource
PreparationBiosimilar diluted in blank serum to create calibration standards
Standard CurveSerial dilutions establish a reference range for quantification
QuantificationStudy samples interpolated from the calibration curve
ValidationAssay demonstrates equivalence of biosimilar versus reference as standard
ApplicationUsed as a standard/control for measuring PK in preclinical or bridging studies

This workflow is essential for accurate, precise, and comparable measurement of obinutuzumab concentrations in serum during PK bridging studies and biosimilar development.

Standard flow cytometry protocols for using a conjugated obinutuzumab biosimilar (such as PE or APC-labeled) to validate CD20 expression or binding typically involve incubation of the antibody with target cells, followed by signal detection and antibody specificity controls.

Overview of Protocol Components

  • Cell Preparation: Isolate single-cell suspensions from relevant samples (e.g., lymphoma cell lines or PBMCs).
  • Antibody Incubation: Incubate the cells with the fluorophore-conjugated obinutuzumab biosimilar (e.g., PE- or APC-labeled) at optimal concentrations and designated temperature (usually 4°C to prevent internalization), typically for 20–30 minutes.
  • Washing: Wash cells to remove unbound antibody.
  • Fixation (Optional): Fixation is sometimes applied, especially if further intracellular staining is needed, although usually not required for CD20 surface analysis.
  • Flow Cytometric Analysis: Analyze the cells by flow cytometry, gating on live lymphocytes and measuring the fluorescence signal corresponding to CD20.

Key Control and Validation Steps

  • Isotype or Conjugate Controls: Use a fluorophore-labeled isotype control or relevant negative control to assess background fluorescence.
  • Competition/Blocking: To confirm specificity, preincubate the sample with an excess of unlabeled obinutuzumab before the labeled biosimilar. A reduction in fluorescence indicates specific binding to CD20.
  • Negative/Low CD20 Expressing Cells: Include CD20-negative cell lines (e.g., CLL-155) or cells known for low expression to define nonspecific binding and lower detection limits.
  • Positive Controls: Use high-CD20-expressing lines (such as Ramos) to confirm the assay’s sensitivity and dynamic range.

Data Interpretation

  • Signal Shift: A strong rightward shift in fluorescence intensity upon incubation with conjugated obinutuzumab indicates binding to CD20-expressing cells.
  • Quantification: Calculate metrics such as mean fluorescence intensity (MFI)^, percentage of positive cells, and, when using standard beads, molecules of equivalent soluble fluorochrome (MESF), to quantify CD20 expression or antibody binding site density.
  • Binding Affinity Estimation: Advanced protocols may include titration curves to estimate binding affinity parameters such as ( K_D ), as exemplified by using ^64Cu-labeled F(ab’)2-obinutuzumab fragments to closely study CD20 binding through flow cytometry.

Reference Protocol Example (Synthesized from ):

  1. Harvest cells and adjust to 1–2×10^6^ cells/mL.
  2. Aliquot 1×10^5^ cells per tube.
  3. Add conjugated obinutuzumab to each tube (suggested: 1–5 μg/mL) and incubate on ice or at 4°C for 30 min.
  4. Wash twice with FACS buffer (PBS with 2% FBS).
  5. (Optional) Fix cells in 1% paraformaldehyde if further analysis is delayed.
  6. Acquire data on a flow cytometer (use appropriate filter for PE or APC).
  7. Analyze for MFI or positive cell percentage.
  8. Include controls as above for data interpretation reliability.

Best Practices

  • Always titrate the antibody to determine optimal concentration for your sample type.
  • Confirm that the conjugated biosimilar retains binding affinity and specificity (should be validated batch-to-batch, particularly for biosimilars).
  • If quantification of receptor density is required, use fluorescent quantification beads for standardization.

These steps and controls are consistent with best practice in flow cytometric assessment of antibody binding and target antigen expression. They have been successfully implemented for evaluating CD20 expression and antibody binding specificity with obinutuzumab, both for basic research and antibody development/characterization purposes.

Biopharma companies typically perform a detailed array of analytical structural and functional assays to confirm that a proposed biosimilar matches the originator drug in both molecular features and biologic function. These studies focus on comparing critical quality attributes (CQAs) using precise and complementary techniques.

Essential context and supporting details:

Core Analytical Assays Typically Performed

  • Structural assays:
    • Primary structure: Peptide mapping and amino acid sequencing to confirm the exact protein sequence.
    • Higher-order structure: Circular dichroism (CD), differential scanning calorimetry (DSC), and spectroscopy for secondary/tertiary structure.
    • Post-translational modifications: Glycosylation analysis, mass spectrometry, and chromatography (HPLC, LC-MS) to detect modifications such as glycosylation, oxidation, or deamidation.
    • Aggregation and purity: Size-exclusion chromatography (SEC), capillary electrophoresis, and dynamic light scattering to detect aggregates, fragments, impurities, and variants.
  • Functional assays:
    • Biological activity: Cell-based assays to assess potency and functional mechanism.
    • Binding assays: Surface plasmon resonance (SPR), ELISA, and affinity measurements for target engagement (e.g., receptor or antigen binding).
    • Enzyme kinetics: When applicable, these confirm the rate and mechanism of enzymatic activity.
    • Fc receptor binding: If relevant, verify affinity to FcγR variants (especially important for monoclonal antibodies).
  • Comparability assessments:
    Head-to-head analysis of the biosimilar against the reference using multiple lots, employing orthogonal methods (different techniques for the same attribute) for precise characterization and to detect even minor differences.

Regulatory Guidance

  • These analyses must meet stringent regulatory standards (such as ICH Q6B), focusing on highly sensitive fingerprinting of CQAs most relevant to clinical performance and safety.

Leinco Biosimilar Use in Analytical StudiesNo specific details regarding the use of a Leinco biosimilar in such analytical similarity studies are indicated in the provided search results. Leinco Technologies is known for supplying recombinant antibodies and biosimilars used as standard references, controls, or assay reagents in analytical testing; in practice, a Leinco biosimilar would be used alongside the reference product for head-to-head comparisons in the described assays—serving either as a sample to verify test performance or as a surrogate standard in specific functional or structural assessment platforms. This is an inference based on general industry practices, given Leinco’s catalog and typical roles as an analytical reagent supplier.

Summary of Key Points

  • Biosimilar analytical similarity assessments rely on a comprehensive suite of structural, physicochemical, and biological functional assays.
  • The outcome must show high similarity in CQAs between the biosimilar and originator drug for regulatory approval.
  • Leinco biosimilars are generally used as standards or controls in these studies, but direct evidence from the search results is lacking. This usage is typical for assay validation and routine comparability assessments, subject to the specific protocols and regulatory guidance.

If further clarity on Leinco biosimilars' specific analytical application is needed, more targeted documentation or direct reference from Leinco would be required.

References & Citations

1. Middleton O, Wheadon H, Michie AM. Classical Complement Pathway. In MJH Ratcliffe (Ed.), Reference Module in Biomedical Sciences Encyclopedia of Immunobiology Volume 2 (pp. 318-324). Elsevier. 2016.
2. Freeman CL, Sehn LH. Br J Haematol. 182(1):29-45. 2018.
3. Mössner E, Brünker P, Moser S, et al. Blood. 115(22):4393-4402. 2010.
4. Chan HT, Hughes D, French RR, et al. Cancer Res. 63(17):5480-5489. 2003.
5. Ivanov A, Beers SA, Walshe CA, et al. J Clin Invest. 119(8):2143-2159. 2009.
6. Alduaij W, Ivanov A, Honeychurch J, et al. Blood. 117(17):4519-4529. 2011.
7. Herter S, Herting F, Mundigl O, et al. Mol Cancer Ther. 12(10):2031-2042. 2013.
8. Honeychurch J, Alduaij W, Azizyan M, et al. Blood. 119(15):3523-3533. 2012.
9. Golay J, Zaffaroni L, Vaccari T, et al. Blood. 95(12):3900-3908. 2000.
10. Cragg MS, Morgan SM, Chan HT, et al. Blood. 101(3):1045-1052. 2003.
11. Cragg MS, Glennie MJ. Blood. 103(7):2738-2743. 2004.
12. Niederfellner G, Lammens A, Mundigl O, et al. Blood. 118(2):358-367. 2011.
13. Klein C, Lammens A, Schäfer W, et al. MAbs. 5(1):22-33. 2013.
14. Könitzer JD, Sieron A, Wacker A, Enenkel B. PLoS One. 10(12):e0145633. 2015.
15. Terszowski G, Klein C, Stern M. J Immunol. 192(12):5618-5624. 2014.
16. Bologna L, Gotti E, Manganini M, et al. J Immunol. 186(6):3762-3769. 2011.
17. Ysebaert L, Laprévotte E, Klein C, Quillet-Mary A. Blood Cancer J. 5(11):e367. 2015.
18. Cartron G, Hourcade-Potelleret F, Morschhauser F, et al. Haematologica. 101(2):226-234. 2016.
Indirect Elisa Protocol
Flow Cytometry

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Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.