Anti-Human CD45RO (Clone UCHL-1) – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Anti-Human CD45RO (Clone UCHL-1) – Purified (PhenoCyler-Fusion (CODEX)® Ready)

Product No.: C503

[product_table name="All Top" skus="C129"]

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Clone
UCHL-1
Target
CD45RO
Formats AvailableView All
Product Type
Monoclonal Antibody
Isotype
IgG2a
Applications
IHC FF
,
IHC FFPE
,
PhenoCycler®

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Product Size
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CODEX® Details

Validation Notes
Clone UCHL-1 has been validated for use on CODEX® using human tonsil formalin-fixed paraffin-embedded (FFPE) and Fresh Frozen (FF) tissue.
Tissue Screened
Tonsil
Tissue Preparation
FF (Fresh Frozen)
FFPE (Formalin-Fixed Paraffin-Embedded)

Antibody and Reporter Details

Reactivity Species
Human
Host Species
Mouse
Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4.
Storage and Handling
This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Applications and Recommended Usage?
Quality Tested by Leinco
CODEX® This CD45RO (Clone UCHL-1) antibody is formulated to simplify the antibody preparation needed when performing a CODEX® barcode conjugate. The suggested concentration is 0.5 mg/ml.
RRID
AB_2893564
Country of Origin
USA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone UCHL-1 recognizes human CD45RO.
Antigen Distribution
The CD45RO antigen is present on most thymocytes, about 40% of resting peripheral blood T-lymphocytes and the majority of T-cells in skin reactive infiltrates and T-cell malignancies. CD45RO is also found on a subset of B-cells and some B-cell lymphomas. NK cells do not express the CD45RO antigen. It is also present on monocytes, macrophages and granulocytes.
Background
CD45 is a 180-240kD glycoprotein member of the protein tyrosine phosphatase (PTP) family known for its involvement in regulating a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45 and its isoforms are vital regulators of T- and B-cell antigen receptor signaling. CD45 functions through its extracellular domain or through its cytoplasmic domain, and serves as a negative regulator of cytokine receptor signaling via JAK kinase supression. The large extracellular domain is highly glycosylated, and its multiple isoforms allow extensive variation in the structure of its side chains. CD45 isoforms show cell-type and differentiation-stage specific expression that can be used as markers that identify and distinguish between different types of immune cells. CD45R is an isoform of CD45 with a molecular weight of 220 kD. CD45R contains all three possible exons (A, B, and C); making it the longest protein generated from alternative splicing with a migration at 200 kD when isolated from T cells. Furthermore, B cells express CD45R with heavier glycosylation, bringing the molecular weight to 220 kD, hence the name B220. Notably, B220 expression is not only restricted to B cells and may also be expressed on activated T cells, on a subset of dendritic cells, and on other antigen-presenting cells. Additionally, activated and memory T lymphocytes express CD45RO which facilitates T cell activation. CD45RO lacks all three possible exons (A, B, and C), making it the shortest CD45 isoform.

Antigen Details

Protein
Ligand/Receptor
CD22
PubMed
NCBI Gene Bank ID

References & Citations

1. Leukocyte Typing IV (1989) Oxford University Press
2. Terry, L. A. et al. (1988) Immunology 64:331
3. Smith, S. H. et al. (1986) Immunology 58:63
4. Norton, A. J. et al. (1986) J. Clin. Pathol. 39:399
IHC
IHC
PhenoCycler®
Products are for research use only. Not for use in diagnostic or therapeutic procedures.