Anti-Human CD64 (FCGR1) – PE

Pricing & Details

Product No.C695
Clone
10.1
Protein
CD64
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
FcγRI, FcR I, Fc Gamma Receptor Ia, Fc-Gamma RIA
Isotype
Mouse
IgG1 κ
Applications
FC
Prod No.
Size
Price
Avail.
Qty
Add to cart
C695-100 µg
100 µg
$192.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Rheumatoid synovial fluid cells and fibronectin purified human monocytes.
Product Concentration
0.2 mg/ml
Formulation
This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This R-phycoerythrin (R-PE) conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Excitation Laser
Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this CD64 antibody, clone 10.1, for staining cells in flow cytometry is ≤ 0.5 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 10.1 recognizes the alpha subunit of human FCGR1.
Antigen Distribution
FCGR1 is expressed on monocytes, macrophages, dendritic cells (DCs), and activated granulocytes.
Background
FCGR1 antibody, 10.1, recognizes high-affinity immunoglobulin gamma Fc receptor I (FCGR1), also known as CD64. FCGR1 is a 72 kDa type I transmembrane glycoprotein expressed on monocytes, macrophages, and dendritic cells (DCs). FCGR1 can also be induced on neutrophils with IFNγ and G-CSF1. FCGR1 binds with high affinity to monomeric IgG1 and IgG3, and to a lesser extent, IgG42, resulting in phosphorylation of the intracellular FCGR1 ITAM motif and subsequent recruitment of Syk. FCGR1 contributes to inflammation via several mechanisms, including promoting antibody-dependent cell-mediated cytotoxicity (ADCC), clearance of immune complexes, cytokine production, and antigen presentation1,3. CD64-based targeted therapies eliminate M1 pro-inflammatory macrophages and show clinical potential for the treatment of macrophage-mediated chronic inflammatory diseases, such as chronic cutaneous inflammation and rheumatoid arthritis4. In addition, CD64 promotes antitumor responses and mediates cytotoxic killing of tumor cells by macrophages5.

Antigen Details

Protein
Ligand/Receptor
IgG receptor
PubMed
NCBI Gene Bank ID
Research Area
Immunology
.
Innate Immunity

References & Citations

1. Hulett MD & Hogarth PM. (1998) Mol Immunol. 35(14-15):989-96
2. M. Daëron., et al. (2009) Blood. 113: 3716–3725
3. Alter G., et al. (2011) Epub. 415(2):160-7
4. Barth S., et al. (2017) Biomedicines. 5(3):56
5. Keler T., et al. (1998) Clin Cancer Res. 4(9):2237-43
Flow Cytometry

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.