Anti-Human CD64 (FCGR1) – Purified in vivo GOLD™ Functional Grade

Anti-Human CD64 (FCGR1) – Purified in vivo GOLD™ Functional Grade

Product No.: I-2000

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Clone
10.1
Target
CD64
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
FcγRI, FcR I, Fc Gamma Receptor Ia, Fc-Gamma RIA
Isotype
IgG1 κ
Applications
B
,
FA
,
FC
,
IHC FF
,
in vivo

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Product Size
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Rheumatoid synovial fluid cells and fibronectin purified human monocytes.
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this CD64 antibody, clone 10.1, for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Additional Applications Reported In Literature ?
B
IHC FF
FA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 10.1 recognizes the alpha subunit of human FCGR1.
Antigen Distribution
FCGR1 is expressed on monocytes, macrophages, dendritic cells (DCs), and activated granulocytes.
Background
FCGR1 antibody, 10.1, recognizes high-affinity immunoglobulin gamma Fc receptor I (FCGR1), also known as CD64. FCGR1 is a 72 kDa type I transmembrane glycoprotein expressed on monocytes, macrophages, and dendritic cells (DCs). FCGR1 can also be induced on neutrophils with IFNγ and G-CSF1. FCGR1 binds with high affinity to monomeric IgG1 and IgG3, and to a lesser extent, IgG42, resulting in phosphorylation of the intracellular FCGR1 ITAM motif and subsequent recruitment of Syk. FCGR1 contributes to inflammation via several mechanisms, including promoting antibody-dependent cell-mediated cytotoxicity (ADCC), clearance of immune complexes, cytokine production, and antigen presentation1,3. CD64-based targeted therapies eliminate M1 pro-inflammatory macrophages and show clinical potential for the treatment of macrophage-mediated chronic inflammatory diseases, such as chronic cutaneous inflammation and rheumatoid arthritis4. In addition, CD64 promotes antitumor responses and mediates cytotoxic killing of tumor cells by macrophages5.

Antigen Details

Ligand/Receptor
IgG receptor
NCBI Gene Bank ID
Research Area
Immunology
.
Innate Immunity

References & Citations

1. Hulett MD & Hogarth PM. (1998) Mol Immunol. 35(14-15):989-96
2. M. Daëron., et al. (2009) Blood. 113: 3716–3725
3. Alter G., et al. (2011) Epub. 415(2):160-7
4. Barth S., et al. (2017) Biomedicines. 5(3):56
5. Keler T., et al. (1998) Clin Cancer Res. 4(9):2237-43
B
FA
Flow Cytometry
IHC FF
in vivo Protocol
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Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.