Anti-Human CXCR4 (Clone 12G5) – Purified in vivo GOLD™ Functional Grade
Anti-Human CXCR4 (Clone 12G5) – Purified in vivo GOLD™ Functional Grade
Product No.: C850
Clone 12G5 Target CXCR4 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Fusin, LESTR, CD184 Isotype Mouse IgG2a k Applications B , FC , ICC , IF Microscopy , IHC , in vivo , N , WB |
Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Recommended Isotype Controls Recommended Dilution Buffer Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity 12G5 activity is directed against human CXCR4 (CD184; Fusin). Background CXCR4 is a G-protein coupled receptor that binds the chemokine CXCL121. Chemokines are small 8-12 kDa proteins that mediate cell migration and arrest, homing and trafficking of leukocytes in bone marrow and lymphoid organs, tissue formation, cytoskeletal rearrangement, and immune cell recruitment to inflammation. Additionally, chemokines are expressed by cancer cells, where they enhance tumor angiogenesis and development. CXCR4 is the chemokine receptor most abundantly expressed2 and most frequently detected3 in various cancer types, being present in malignant cell subpopulations in primary tumors as well as sites of metastasis. CXCR4 is involved in tumor cell proliferation and migration2 and is involved in leukocyte chemotaxis in several autoimmune diseases1. CXCR4 also acts as an alternative receptor for some isolates of HIV-2 in the absence of CD44. CXCR4 expression is regulated by HIF-1α, IL-5, IFN-γ, TGF-β, and IL-17A1.
12G5 was produced by immunizing Balb/c mice with CP-MAC-infected Sup-T1 cells4. Hybridomas were generated and screened for the ability to inhibit CP-MAC-induced syncytium induction on Sup-T1 cells. 12G5 binds specifically to both human and nonhuman cells that express recombinant CXCR44. 12G5 inhibits CD4-independent infection by some HIV-2 isolates, and preincubating cells with 12G5 abolishes syncytium formation. HIV-2/vcp-infected cells display a marked and selective reduction in 12G5 binding. 12G5 also inhibits induction of cell-to-cell fusion of CXCR4+ RD/CD4 cells by HIV-1 and HIV-2 strains5. Antigen Distribution CXCR4 is expressed in various organs including ovary, bone marrow, kidney, lung, small intestine, spleen, lymph nodes, brain, stomach, liver, thymus, heart, and pancreas as well as on the surface of endothelial mature and precursor cells and pericytes. PubMed NCBI Gene Bank ID UniProt.org Research Area Immunology Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Use of Clone 12G5 (Anti-CXCR4/CD184) in In Vivo Mouse StudiesSummary Key In Vivo ApplicationsIn Vivo Imaging and Biodistribution Studies Mechanism and Rationale Clinical and Translational ImplicationsFunctional Blockade and Therapeutic Potential Technical ConsiderationsSpecies Specificity Delivery and Monitoring
Summary Table: In Vivo Applications of Clone 12G5 in Mice
ConclusionClone 12G5 is primarily used in in vivo mouse studies involving human tumor xenografts, where it serves as a specific probe for human CXCR4 expression and distribution via radiolabeled imaging. Its application is restricted to models with human cells due to species specificity. While it has shown promise in biodistribution and imaging studies, its direct therapeutic or functional blockade potential in vivo remains a subject for future investigation. Storage Temperature for Sterile Packaged Clone 12G5Optimal Storage Conditions Clone 12G5 is a widely used CXCR4 monoclonal antibody. The correct storage temperature depends on the formulation and manufacturers instructions.
Key Considerations
Summary Table
Always follow the product-specific guidelines provided with your antibody clone 12G5 for best stability and performance. Alongside 12G5, which targets CXCR4 (CD184), the literature commonly pairs it with other antibodies or proteins that recognize CCR5 (CD195) and their ligands, especially in studies related to HIV entry, chemokine receptor biology, and immune cell subset identification. Frequently used in combination with 12G5 are:
These antibodies and proteins together allow:
Additional combinations can depend on cell type or context (e.g., B cell, T cell, or stem cell markers), but 2D7 and PRO 140 are the most consistently co-cited protein reagents with 12G5 in immunology and HIV literature. The monoclonal antibody 12G5 is a significant tool in scientific research, particularly noted for its specificity against the human CXCR4 receptor. Here are the key findings from scientific literature regarding clone 12G5:
In summary, 12G5 is a valuable tool for studying CXCR4-mediated processes, particularly in the context of HIV infection and cell signaling, and provides insights into the interaction between CXCR4 and its antagonists. References & Citations1. Mousavi A. Immunol Lett. 217:91-115. 2020.
2. Barbieri F, Bajetto A, Thellung S, et al. Expert Opin Drug Discov. 11(11):1093-1109. 2016. 3. Bajetto A, Barbieri F, Dorcaratto A, et al. Neurochem Int. 49(5):423-432. 2006. 4. Endres MJ, Clapham PR, Marsh M, et al. Cell. 87(4):745-756. 1996. 5. McKnight A, Wilkinson D, Simmons G, et al. J Virol. 71(2):1692-1696. 1997. 6. Fischer T, Nagel F, Jacobs S, et al. PLoS One. 3(12):e4069. 2008. 7. Volin MV, Joseph L, Shockley MS, et al. Biochem Biophys Res Commun. 242(1):46-53. 1998. 8. Berndt C, Möpps B, Angermüller S, et al. Proc Natl Acad Sci U S A. 95(21):12556-12561. 1998. 9. Ullrich CK, Groopman JE, Ganju RK. Blood. 96(4):1438-1442. 2000. 10. Murga M, Fernandez-Capetillo O, Tosato G. Blood. 105(5):1992-1999. 2005. Technical ProtocolsCertificate of Analysis |
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