Anti-Human CXCR4 (Clone 12G5)(Sino Target) – Purified in vivo GOLD™ Functional Grade

Home/Bulk In vivo Functional Grade Antibodies, Gold Purified Bulk Antibodies, Primary Monoclonal Antibodies/Anti-Human CXCR4 (Clone 12G5)(Sino Target) – Purified in vivo GOLD™ Functional Grade

Pricing & Details

Product No.C850
Clone
12G5
Protein
CXCR4
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Fusin, LESTR, CD184
Isotype
Mouse
IgG2a κ
Applications
B
,
FC
,
ICC
,
IF Microscopy
,
IHC
,
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C850-1.0mg
1.0 mg
$105.00
In stock
Max:
Min: 1
Step: 1
C850-5.0mg
5.0 mg
$355.00
In stock
Max:
Min: 1
Step: 1
C850-25mg
25 mg
$965.00
In stock
Max:
Min: 1
Step: 1
C850-50mg
50 mg
$1,595.00
In stock
Max:
Min: 1
Step: 1
C850-100mg
100 mg
$2,300.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
12G5 activity is directed against human CXCR4 (CD184; Fusin).
Antigen Distribution
CXCR4 is expressed in various organs including ovary, bone marrow, kidney, lung, small intestine, spleen, lymph nodes, brain, stomach, liver, thymus, heart, and pancreas as well as on the surface of endothelial mature and precursor cells and pericytes.
Background
CXCR4 is a G-protein coupled receptor that binds the chemokine CXCL121. Chemokines are small 8-12 kDa proteins that mediate cell migration and arrest, homing and trafficking of leukocytes in bone marrow and lymphoid organs, tissue formation, cytoskeletal rearrangement, and immune cell recruitment to inflammation. Additionally, chemokines are expressed by cancer cells, where they enhance tumor angiogenesis and development. CXCR4 is the chemokine receptor most abundantly expressed2 and most frequently detected3 in various cancer types, being present in malignant cell subpopulations in primary tumors as well as sites of metastasis. CXCR4 is involved in tumor cell proliferation and migration2 and is involved in leukocyte chemotaxis in several autoimmune diseases1. CXCR4 also acts as an alternative receptor for some isolates of HIV-2 in the absence of CD44. CXCR4 expression is regulated by HIF-1α, IL-5, IFN-γ, TGF-β, and IL-17A1.

12G5 was produced by immunizing Balb/c mice with CP-MAC-infected Sup-T1 cells4. Hybridomas were generated and screened for the ability to inhibit CP-MAC-induced syncytium induction on Sup-T1 cells.

12G5 binds specifically to both human and nonhuman cells that express recombinant CXCR44. 12G5 inhibits CD4-independent infection by some HIV-2 isolates, and preincubating cells with 12G5 abolishes syncytium formation. HIV-2/vcp-infected cells display a marked and selective reduction in 12G5 binding. 12G5 also inhibits induction of cell-to-cell fusion of CXCR4+ RD/CD4 cells by HIV-1 and HIV-2 strains5.

Antigen Details

Protein
PubMed
NCBI Gene Bank ID
Research Area
Immunology

References & Citations

1. Mousavi A. Immunol Lett. 217:91-115. 2020.
2. Barbieri F, Bajetto A, Thellung S, et al. Expert Opin Drug Discov. 11(11):1093-1109. 2016.
3. Bajetto A, Barbieri F, Dorcaratto A, et al. Neurochem Int. 49(5):423-432. 2006.
4. Endres MJ, Clapham PR, Marsh M, et al. Cell. 87(4):745-756. 1996.
5. McKnight A, Wilkinson D, Simmons G, et al. J Virol. 71(2):1692-1696. 1997.
6. Fischer T, Nagel F, Jacobs S, et al. PLoS One. 3(12):e4069. 2008.
7. Volin MV, Joseph L, Shockley MS, et al. Biochem Biophys Res Commun. 242(1):46-53. 1998.
8. Berndt C, Möpps B, Angermüller S, et al. Proc Natl Acad Sci U S A. 95(21):12556-12561. 1998.
9. Ullrich CK, Groopman JE, Ganju RK. Blood. 96(4):1438-1442. 2000.
10. Murga M, Fernandez-Capetillo O, Tosato G. Blood. 105(5):1992-1999. 2005.
Flow Cytometry
IHC
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.