Anti-Human FGF-6 (Clone 27028)

Anti-Human FGF-6 (Clone 27028)

Product No.: F1067

[product_table name="All Top" skus="F1067"]

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Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Fibroblast Growth Factor-6, HBGF-6, HST-2

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Antibody Details

Product Details

Reactive Species
Host Species
Purified Recombinant Human FGF-6 (Accession # P10767)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 1-2 µg/ml. A suitable detection antibody is PN:F1092 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:F121.
Additional Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human FGF-6 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human FGF-6 (at 1 ng/ml and in the presence of 1.0 μg/mL of heparin, using the FGF-6 responsive NR6R-3T3 fibroblasts as target cells.) is 1.0 - 4.0 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.


Mouse Anti-Human Fibroblast Growth Factor 6 (FGF-6) (Clone 27028) recognizes an epitope on Human FGF-6. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody will not neutralize the activity of FGF acidic, FGF basic, rhFGF-4 or rhFGF-5. Based on direct ELISA, this antibody shows approximately 75% cross-reactivity with rhFGF-5 and less than 10% cross-reactivity with rhFGF-4.
Fibroblast growth factor 6, also known as FGF6, hst-2 or hst-1-related is a member of the fibroblast growth factor (FGF) family. 1,2 FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF6 is the only known member of the FGF family whose expression is restricted to the muscle cell lineage during development, suggesting it may have a role in myogenesis.3 FGF6 has a dual function in muscle regeneration, stimulating myoblast proliferation/migration and muscle differentiation/hypertrophy.4

Antigen Details


References & Citations

1. Iida, S. et al. (1992) Oncogene 7: 303
2. Marics, I. et al. (1989) Oncogene 4: 335
3. DeLapeyrière, O. et al. (1996) Exp Cell Res. 224: 143
4. Chanoine, C. et al. (2006) Biochim Biophys Acta 1763: 773
Elisa Sandwich Protocol
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Formats Available

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Prod No.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.