Anti-Human FGF-Acidic (Clone 3117)
Pricing & Details
Purified Recombinant Human FGF Acidic (>98%)
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human FGF Acidic this monoclonal antibody can be used at a concentration of 1 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human FGF Acidic is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:E193.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Mouse Anti-Human FGF acidic (FGF acidic) (Clone 3117) recognizes an epitope on Human FGF acidic. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Fibroblast growth factor 1 (acidic), also known as FGF1, is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-1 is the only member of the FGF family that binds with high affinity to all four FGF receptors.1FGF1 plays an important role in hematopoietic stem cell homeostasis. The FGF pathway regulates primitive hematopoiesis by modulating transcription factors such as Gata1 expression level and activity.2
References & Citations
1. Jaye, M. et al. (1986) Science 233:541
2. Otlewski, J. et al. (2009) Acta. Crystallogr. D. Biol. Crystallogr. 65:67
3. Nakazawa, F. et al. (2006) Blood. 108:3335
4. Bjornsson, TD. et al. (1991) Proc. Natl. Acad. Sci. (USA) 88:8651
5. Merwin, JR. et al. (1992) Cancer Res. 52:4995
Products are for research use only. Not for use in diagnostic or therapeutic procedures.