Anti-Human FGF-Acidic (Clone 3117)
Anti-Human FGF-Acidic (Clone 3117)
Product No.: F1065
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Clone 3117 Target FGF-Acidic Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Fibroblast Growth Factor-Acidic, FGF-1, ECGF, HBGF-1, AFGF, ECGF-Beta, ECGFA, ECGFB, FGF-Alpha, FGFA, GLIO703 Isotype IgG1 Applications WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Purified Recombinant Human FGF Acidic (>98%) Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830109 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human FGF Acidic this monoclonal antibody can be used at a concentration of 1 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human FGF Acidic is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:E193. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Human FGF acidic (FGF acidic) (Clone 3117) recognizes an epitope on Human FGF acidic. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Fibroblast growth factor 1 (acidic), also known as FGF1, is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-1 is the only member of the FGF family that binds with high affinity to all four FGF receptors.1FGF1 plays an important role in hematopoietic stem cell homeostasis. The FGF pathway regulates primitive hematopoiesis by modulating transcription factors such as Gata1 expression level and activity.2 Antigen DetailsPubMed References & Citations1. Jaye, M. et al. (1986) Science 233:541 2. Otlewski, J. et al. (2009) Acta. Crystallogr. D. Biol. Crystallogr. 65:67 3. Nakazawa, F. et al. (2006) Blood. 108:3335 4. Bjornsson, TD. et al. (1991) Proc. Natl. Acad. Sci. (USA) 88:8651 5. Merwin, JR. et al. (1992) Cancer Res. 52:4995 Technical Protocols |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
