Anti-Human FGF-Acidic (Clone 3117)

Anti-Human FGF-Acidic (Clone 3117)

Product No.: F1065

[product_table name="All Top" skus="F1065"]

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Clone
3117
Target
FGF-Acidic
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Fibroblast Growth Factor-Acidic, FGF-1, ECGF, HBGF-1, AFGF, ECGF-Beta, ECGFA, ECGFB, FGF-Alpha, FGFA, GLIO703
Isotype
IgG1
Applications
WB

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human FGF Acidic (>98%)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human FGF Acidic this monoclonal antibody can be used at a concentration of 1 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human FGF Acidic is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:E193.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human FGF acidic (FGF acidic) (Clone 3117) recognizes an epitope on Human FGF acidic. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Fibroblast growth factor 1 (acidic), also known as FGF1, is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion.
FGF-1 is the only member of the FGF family that binds with high affinity to all four FGF receptors.1

FGF1 plays an important role in hematopoietic stem cell homeostasis. The FGF pathway regulates primitive hematopoiesis by modulating transcription factors such as Gata1 expression level and activity.2

Antigen Details

References & Citations

1. Jaye, M. et al. (1986) Science 233:541
2. Otlewski, J. et al. (2009) Acta. Crystallogr. D. Biol. Crystallogr. 65:67
3. Nakazawa, F. et al. (2006) Blood. 108:3335
4. Bjornsson, TD. et al. (1991) Proc. Natl. Acad. Sci. (USA) 88:8651
5. Merwin, JR. et al. (1992) Cancer Res. 52:4995
General Western Blot Protocol

Formats Available

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Prod No.
Description
F1065
Products are for research use only. Not for use in diagnostic or therapeutic procedures.