Anti-Human GITRL

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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Rabbit Anti-Human GITRL recognizes Human and Mouse GITRL. This polyclonal antibody was purified using affinity chromatography.

The tumor necrosis factor (TNF) and TNF receptor (TNFR) gene superfamilies regulate numerous biological functions including cell proliferation, differentiation, and survival through regulating the activation of the transcription factor NF-κB and various mitogen-activated protein kinases (reviewed in 1). The glucocorticoid-induced tumor necrosis factor receptor (GITR) is an emerging member of this family that is expressed on CD4+ CD25+ regulatory T cells and appears to have crucial immune regulation functions.^2,3 Its ligand GITRL is expressed in endothelial and antigen-presenting cells⁴ and can activate NF-κB, induce both pro- and anti-apoptotic effects, inhibit the suppressive activity of regulatory T cells, and co-stimulate responder T cells through GITR.⁵ Dominant negative forms of NIK and TRAF2 expressed in transfected 293 cells substantially inhibited NF-κB activation, suggesting that the GITRL-GITR pathway involves both NIK and TRAF2.⁴

1. Gaur, U. et al. (2003) Biochem. Pharmacol. 66:1403
2. Ronchetti, S. et al (2002) Blood 100:350
3. Shimizu, J. et al. (2002) Nat. Immunol. 3:135
4. Gurney, AL. et al. (1999) Curr. Biol. 9:215