Anti-Human IL-19

Product No.: I-871


Clone 152112 Target IL-19 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Interleukin-19, Melanoma differentiation association like protein Isotype IgG2b Applications ELISA Indirect , FC , N


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Data


Antibody Details Product Details Reactive Species Human Host Species Mouse Immunogen Purified Recombinant Human IL-19 (Accession # AAN40906) Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830957 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing IL-19. A suggested method would be to stain cells expressing IL-19 with 2.5 µg/10⁶ cells in a total staining volume of 200 µl followed by PN:M1189. Neutralization: This antibody is useful for neutralization of Human IL-19 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human IL-19 (at 3 ng/ml) is 0.2-0.5 µg/ml. Additional Applications Reported In Literature ? Neutralization: Measured by its ability to neutralize IL‑19-induced proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑20 R alpha and IL‑20 R beta. The Neutralization Dose (ND50) is typically 0.2-0.5 µg/mL in the presence of 3 ng/mL Recombinant Human IL‑19. CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity Mouse Anti-Human Interleukin 19 (IL-19) (Clone 152112) recognizes an epitope on Human IL-19. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. PubMed IL-19 NCBI Gene Bank ID 29949 UniProt.org Information on Uniprot.org References & Citations View product citations for antibody I-871 on CiteAb Technical Protocols N Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Mouse

Immunogen

Purified Recombinant Human IL-19 (Accession # AAN40906)

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2830957

Applications and Recommended Usage?
Quality Tested by Leinco

Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing IL-19. A suggested method would be to stain cells expressing IL-19 with 2.5 µg/10⁶ cells in a total staining volume of 200 µl followed by PN:M1189.

Neutralization: This antibody is useful for neutralization of Human IL-19 bioactivity. The antibody dose required to neutralize 50% (ND₅₀) of the biological activity of Human IL-19 (at 3 ng/ml) is 0.2-0.5 µg/ml.

Additional Applications Reported In Literature ?

Neutralization: Measured by its ability to neutralize IL‑19-induced proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑20 R alpha and IL‑20 R beta. The Neutralization Dose (ND50) is typically 0.2-0.5 µg/mL in the presence of 3 ng/mL Recombinant Human IL‑19.
CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human Interleukin 19 (IL-19) (Clone 152112) recognizes an epitope on Human IL-19. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

PubMed

IL-19

NCBI Gene Bank ID

29949

UniProt.org

Information on Uniprot.org