Anti-Human IRE1p (CT)

Pricing & Details

Product No.I-462
Protein
IRE1p
Formats AvailableView All
Product Type
Polyclonal Antibody
Alternate Names
Endoplasmic Reticulum-to-Nucleus Signaling 1, ERN1
Applications
IHC FFPE
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
I-462-20ug
20 µg
$195.00
In stock
Max:
Min: 1
Step: 1
I-462-0.1 mg
0.1 mg
$397.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Data

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Rabbit
Immunogen
PN:I-484
Product Concentration
0.5 mg/ml
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Handling
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day Ambient
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Anti-Human IRE1p recognizes an epitope near the C-terminus of Human, Mouse and Rat IRE1p. This polyclonal antibody was purified using affinity chromatography.
Background
Accumulation of malfolded proteins in the endoplasmic reticulum (ER) activates the unfolded protein response (UPR) and the upregulation of the ER molecular chaperones GRP78 and GRP 94.1,2 These proteins are normally bound to ER transmembrane proteins such as IRE1p and ATF63,4 but ER stress causes their dissociation. This allows IRE1p, a serine-threonine protein kinase to transduce the unfolded protein signal from the ER to the nucleus. IRE1p also has an endoribonuclease activity that is required to splice X-box binding protein (XBP1) mRNA converting it to a potent UPR transcriptional activation. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Antigen Details

Protein
PubMed

References & Citations

1. Takeda, K. et al. (2003) Annu. Rev. Immunol. 21:335
2. Lee, AS. (2005) Methods 35:373
3. Bertolotti, A. et al. (2000) Nat. Cell Biol. 2:326
4. Shen, J. et al. (2002) Dev. Cell 3:99
5. Lee, K. et al. (2002) Genes Dev. 16:452
6. Miyoshi, K. et al. (2000) Res. Mol. Brain Res. 85:68
IHC
General Western Blot Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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