IRE1p (Intermediate Domain) Blocking Peptide

15 amino acids near the center of human IRE1p.

This peptide is formulated in PBS pH 7.2 (0.01 M Sodium Phosphate, 0.13 M NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide.

Store this peptide in working aliquots at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Next Day Ambient

15 amino acids near the center of human IRE1p.

IRE1p peptide is applicable as a control peptide for blocking antibody binding in Western blotting.

1. Miyoshi K, Katayama T, Imaizumi K, et al. Characterization of mouse Ire1 alpha: cloning, mRNA localization in the brain and functional analysis in a neural cell line. Brain Res. Mol. Brain Res. 2000; 85:68-76. 2. Janeway CA Jr. and Medzhitov R. Innate immune recognition. Annu. Rev. Immunol. 2002; 20:197-216. 3. McGettrick AF and O’Neill LAJ. The expanding family of MyD88-like adaptors in Toll-like receptor signal transduction. Mol. Imm. 2004; 41:577-82. 4. Hayashi F, Smith KD, Ozinsky A, et al. The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5. Nature 2001; 410:1099-103. 5. Hawn TR, Verbon A, Lettinga KD, et al. A common dominant TLR5 stop codon polymorphism abolishes flagellin signaling and is associated with susceptibility to legionnaires’ disease. J. Exp. Med. 2003; 198:1563-72.