Anti-Human/Mouse/Rat Activin A βA Subunit

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Pricing & Details

Product No.A374
Clone
69403
Protein
Activin A
Formats AvailableView All
Product Type
Monoclonal Antibody
Isotype
Mouse
IgG1
Applications
ELISA Cap
,
IHC FFPE
,
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
A374-25 µg
25 µg
$205.00
In stock
Max:
Min: 1
Step: 1
A374-100 µg
100 µg
$421.00
In stock
Max:
Min: 1
Step: 1
A374-500 µg
500 µg
$917.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human/Mouse/Rat
Host Species
Mouse
Immunogen
Purified Recombinant Human Activin A (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml.
Other Applications Reported In Literature ?
Neutralization: This antibody is useful for neutralization of Human/Mouse/Rat Activin A bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human/Mouse/Rat Activin A (at 0.75 ng/ml) is 0.02 - 0.06 µg/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 69403 recognizes an epitope on Human Activin A. This antibody will also detect the mature βA subunit from mouse and rat because the amino acid sequences of mature βA subunits from human, mouse and rat are identical. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Activin A is a member of the TGF beta super family. It was originally identified and isolated because it stimulates the release of follicle-stimulating hormone (FSH). Activins are structurally related to inhibins, they share common beta subunits, but they exhibit opposite biological activities. Activins are homodimers of beta subunits and inhibins are heterodimers of an alpha subunit and a beta subunit. Since the initial discovery of Activin A, it has been shown to regulate cell differentiation, nerve cell survival, bone growth promotion, cell proliferation, and apoptosis. Activin A expression has been detected in prostate cancer, colon cancer and breast cancer. Because Activin A is able to inhibit cell proliferation by modulating the Rb pathway, it is thought to inhibit cancer development. This may not be the only mechanism by which Activin may play a preventative role in cancer development. Activin has also been shown to increase the expression of a protein whose decreased expression is correlated with increased metastatic capacity. In addition, Activin has an inhibitory effect on endothelial cell growth and can thereby block angiogenesis, preventing tumor growth and metastasis.

The Activin/Inhibin nomenclature reflects the subunit composition of the proteins: Activin A (βA - βA), Activin B (βB - βB), Activin AB (βA - βB), Inhibin A (α - βA) and Inhibin B (α - βB). At present, little is known about the contribution of the other β subunits to Activin or Inhibin formation and biology. At the amino acid sequence level, the mature human βA subunit is 100% identical to mouse βA, while the human and mouse α subunits share approximately 80% identity. Similarly to other TGF-β family members, Activins exert their biological activities through binding to the heterodimeric complex composed of two membrane spanning serine-threonine kinases designated type I and type II. Two forms of Activin receptor type I (Act RI-A and Act RI-B) and two forms of activin receptor type II (Act RII-A and Act RII-B) have been identified. Activin binds directly to Act RII, the complex then associates with Act RI and initiates signaling.

Antigen Details

Protein
PubMed
Elisa Sandwich Protocol
IHC
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.