Anti-Human Oncostatin M – Biotin
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Antibody DetailsProduct DetailsReactive Species Human Host Species Goat Immunogen Purified Recombinant Human OSM (Accession # P13725) Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831487 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing OSM. A suggested method would be to stain cells expressing OSM with 3 - 10 µg per 1 x 106 cells in a total staining volume of 100 µl followed by PN:A104. Western Blotting: To detect Human OSM this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human OSM is 2 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:O101. ELISA Detection: Suitable for use at concentration of 0.1-0.4 µg/mL . Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Goat Anti-Human Oncostatin M (OSM) recognizes Human OSM. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Oncostatin M (OSM) is a pleitropic cytokine produced by activated T lymphocytes and monocytes that is structurally and functionally related to the subfamily of cytokines known as the IL-6-type cytokine family.1 OSM either inhibits or enhances the growth of cells depending on cell type. It inhibits proliferation of skeletal muscle cells by blocking cell cycle progression from G(1) to S phase.2 OSM is also a key mediator for proliferation and anti-apoptosis of hepatocytes which suggests that OSM gene therapy is useful, as preventive and curative means, for the treatment of patients with liver damage.3 PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Gómez-Lechón, MJ. et al. (1999) Life Sci. 65: 2019 2. Jo, SA. et al. (2008) Cell Signal 20: 120 3. Tsujimura, T. et al. (2007) Am J Pathol. 171: 872 Technical ProtocolsELISA Det   Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
