Anti-Human Oncostatin M (Clone 17001)
Anti-Human Oncostatin M (Clone 17001)
Product No.: O126
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Clone 17001 Target Oncostatin M Formats AvailableView All Product Type Monoclonal Antibody Isotype IgG2a Applications ELISA Cap , N , WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Purified Recombinant Human OSM (Accession # P13725) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831493 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:O103 at a concentration of approximately 30 ng/ml. A suggested standard for this assay is PN:O101. Western Blotting: To detect Human OSM this monoclonal antibody can be used at a concentration of 1 - 2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human OSM is 50 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:O101. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Human OSM bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human OSM (at 2.0 ng/ml) is 0.5 - 2.0 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Mouse Anti-Human Oncostatin M (OSM) (Clone 17001) recognizes an epitope on Human OSM. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Oncostatin M (OSM) is a pleitropic cytokine produced by activated T lymphocytes and monocytes that is structurally and functionally related to the subfamily of cytokines known as the IL-6-type cytokine family.1 OSM either inhibits or enhances the growth of cells depending on cell type. It inhibits proliferation of skeletal muscle cells by blocking cell cycle progression from G(1) to S phase.2 OSM is also a key mediator for proliferation and anti-apoptosis of hepatocytes which suggests that OSM gene therapy is useful, as preventive and curative means, for the treatment of patients with liver damage.3 Antigen DetailsPubMed References & Citations1. Gómez-Lechón, MJ. et al. (1999) Life Sci. 65: 2019 2. Jo, SA. et al. (2008) Cell Signal 20: 120 3. Tsujimura, T. et al. (2007) Am J Pathol. 171: 872 Technical Protocols N  |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
