Anti-Human PDGF-B subunit

Pricing & Details

Product No.P273
Clone
108104
Protein
PDGF-B
Formats AvailableView All
Product Type
Monoclonal Antibody
Isotype
Mouse
IgG2b
Applications
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
P273-25 µg
25 µg
$185.00
In stock
Max:
Min: 1
Step: 1
P273-100 µg
100 µg
$349.00
In stock
Max:
Min: 1
Step: 1
P273-500 µg
500 µg
$920.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human PDGF-B (>98%)
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blotting: To detect Human PDGF-B this monoclonal antibody can be used at a concentration of 1 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:P126.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human Platelet-Derived Growth Factor BB (PDGF-B) (Clone 108104) recognizes an epitope on Human PDGF-B. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody shows approximately 100% cross-reactivity with rhPDGF-AB and rrPDGF-BB, 15% cross-reactivity with rhPDGF-AA and no cross-reactivity with rhPlGF, rhVEGF, rhVEGF-C, rhVEGF-D, rhCTGF and rhLDGF.
Background
PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet alpha-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB.

Antigen Details

Protein
PubMed

References & Citations

1. Pullen, N. et al. (2015) Biochemistry.54(10):1918-29.
2. Wei, Q. et al. (2018) Int J Cancer. 142(7):1322-1331.
General Western Blot Protocol
Prod No.
Description
P273

Formats Available

Prod No.
Description
P273
Products are for research use only. Not for use in diagnostic or therapeutic procedures.