Anti-Human PIST (Intermediate Domain)

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Pricing & Details

Product No.P214
Protein
PIST
Formats AvailableView All
Product Type
Polyclonal Antibody
Alternate Names
Golgi Associated PDZ and Coiled-coil Motif Containing/GOPC
Applications
IHC FFPE
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
P214-20ug
20 µg
$195.00
In stock
Max:
Min: 1
Step: 1
P214-0.1 mg
0.1 mg
$397.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Data

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Rabbit
Immunogen
PN:P224
Product Concentration
0.5 mg/ml
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Handling
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day Ambient
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Anti-Human PIST recognizes an epitope in the intermediate domain of Human and Mouse PIST. This polyclonal antibody was purified using affinity chromatography.
Background
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components1,2 and is negatively regulated by TOR (Target of rapamycin).3 PIST, a PDZ-containing protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6.4 Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1.

Antigen Details

Protein
PubMed

References & Citations

1. Gozuacik, D. et al. (2004) Oncogene 23:2891
2. Kisen, GO. et al. (1993) Carcinogenesis 14:2501
3. Kamada, Y. et al. (2000) J. Cell. Biol. 150:1507
4. Yu, Z. et al. (2002) Neuron 35:921
IHC
General Western Blot Protocol
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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