Anti-Human PIST (Intermediate Domain)

PN:P224

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Next Day Ambient

Rabbit Anti-Human PIST recognizes an epitope in the intermediate domain of Human and Mouse PIST. This polyclonal antibody was purified using affinity chromatography.

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components^1,2 and is negatively regulated by TOR (Target of rapamycin).³ PIST, a PDZ-containing protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6.⁴ Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1.

1. Gozuacik, D. et al. (2004) Oncogene 23:2891
2. Kisen, GO. et al. (1993) Carcinogenesis 14:2501
3. Kamada, Y. et al. (2000) J. Cell. Biol. 150:1507
4. Yu, Z. et al. (2002) Neuron 35:921