This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Handling
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day Ambient
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Rabbit Anti-Human Suppressor of Cytokine Signaling 1 (SOCS1) recognizes an epitope near the C-terminus of Human, Mouse and Rat SOCS1. This polyclonal antibody was purified using affinity chromatography.
The Suppressor of cytokine signaling (SOCS) and cytokine-inducible SH2 proteins are a family of intracellular proteins which regulate the immune cell responses to cytokines.1 SOCS1 acts to suppress dendritic cell (DC) as well as T cell hyperactivation following cytokine signaling by inhibiting JAK tyrosine kinase, a kinase necessary for type I and II cytokine receptors to initiate signaling, by directly binding to the catalytic domain of the kinase.2 SOCS1 also possesses E3 ubiquitin protein ligase activity that results in the polyubiquitination of its target proteins and subsequent degradation by the proteosome.3 It is through this method that SOCS1 negatively regulates signaling by Toll-like receptors TLR2 and TLR4 by mediating the degradation of the TLR signaling adaptor protein TIRAP.4
References & Citations
1. Rakesh, K. et al. (2005) Biochem. Pharm. 70:649
2. O’Shea, JJ. et al. (2002) Cell 109:S121
3. Kile, BT. et al. (2002) Trends Biochem. Sci. 27:235
4. Mansell, A. et al. (2006) Nat. Immunol. 7:148
Products are for research use only. Not for use in diagnostic or therapeutic procedures.