This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Handling
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day Ambient
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Rabbit Anti-Human X Box Binding Protein 1 (XBP-1) recognizes an epitope near the N-terminus of Human XBP-1. This polyclonal antibody was purified using affinity chromatography.
X box binding protein 1 (XBP-1) is a key protein in the mammalian unfolded protein response (UPR) that protects the cell against the stress of malfolded proteins in the endoplasmic reticulum (ER) . Upon sensing unfolded proteins, an ER transmembrane endonuclease and kinase termed IRE1p is activated and excises an intron from XBP-1 mRNA. The spliced XBP-1 mRNA results in a 371 amino acid protein (XBP-1s) which is then translocated to the nucleus where it binds to the regulatory elements of downstream genes. Together with other UPR transcription factors such as ATF6, XBP-1 stimulates the production of ER stress proteins including the ER resident protein chaperones glucose regulated protein (GRP) 78 and GRP94.3,4
References & Citations
1. Yoshida, H. et al. (2001) Cell 107:881
2. Calfon, M. et al. (2002) Nature 415:92
3. Haze, K. et al. (1999) Mol. Cell. Biol. 10:3787
4. Little, E. et al. (1994) Crit. Rev. Eukaryot. Gene Expr. 4:1
Products are for research use only. Not for use in diagnostic or therapeutic procedures.