Anti-Human XAF-1 (Intermediate Domain)

PN:X107

This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.

This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

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Rabbit Anti-Human XAF-1 recognizes an epitope in the intermediate domain of Human XAF-1. This polyclonal antibody was purified using affinity chromatography.

XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and –9, and triggers its relocation from the cytosol to the nucleus.^1,2 Overexpression of XAF-1 results in the neutralization of XIAP’s ability to inhibit cell death.¹ XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines.³ In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF-1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines.^2,3 Both IFN-α2 and IFN-β can induce XAF-1 mRNA in all cells examined but induction of XAF-1 protein (as observed by immunoblot analysis) was seen only in cell lines sensitive to the apoptotic effects of IFNs.⁴

1. Liston, P. et al. (2001) Nature Cell Biol. 3:128
2. Deveraux, QL. et al. (1997) Nature 388:300
3. Fong, WG. et al. (2000) Genomics 70:113
4. Leaman, DW. et al. (2002) J. Biol. Chem. 277:28504