This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Handling
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day Ambient
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Rabbit Anti-Human XAF-1 recognizes an epitope near the C-terminus of Human and Mouse XAF-1. This polyclonal antibody was purified using affinity chromatography.
XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and -9, and triggers its relocation from the cytosol to the nucleus.1,2 Overexpression of XAF-1 results in the neutralization of XIAP's ability to inhibit cell death.1 XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines.3 In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF-1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines.2,3 Both IFN-α2 and IFN-β can induce XAF-1 mRNA in all cells examined but induction of XAF-1 protein (as observed by immunoblot analysis) was seen only in cell lines sensitive to the apoptotic effects of IFNs.4
References & Citations
1. Liston, P. et al. (2001) Nature Cell Biol. 3:128
2. Deveraux, QL. et al. (1997) Nature 388:300
3. Fong, WG. et al. (2000) Genomics 70:113
4. Leaman, DW. et al. (2002) J. Biol. Chem. 277:28504
Products are for research use only. Not for use in diagnostic or therapeutic procedures.