Anti-Influenza A, HA (Clone: FluA-20)

Pricing & Details

Product No.LT578
Clone
FluA-20
Protein
Flu A, HA
Formats AvailableView All
Product Type
Recombinant Monoclonal Antibody
Alternate Names
FluA
Isotype
Human IgG1
Applications
ELISA
,
FC
Prod No.
Size
Price
Avail.
Qty
Add to cart
LT578-250 µg
250 µg
$390.00
Pre-order
Max:
Min: 1
Step: 1
LT578-1.0 mg
1.0 mg
$1,400.00
Pre-order
Max:
Min: 1
Step: 1
LT578-5 mg
5 mg
$5,250.00
Pre-order
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Influenza A Virus
Virus
Expression Host
HEK-293
Immunogen
FluA-20 was generated from peripheral blood samples obtained from a vaccinated donor1.
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
Formulation
This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal-free facility using only In vitro protein-free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A, or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one year. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≥ -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Standard Overnight on Blue Ice.
Other Applications Reported In Literature ?
ELISA
FC
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
FluA-20 activity is directed against a novel epitope at the trimer interface of the hemagglutinin (HA) head domain of most influenza A viruses. Furthermore this antibody binds to HA trimers from a wide array of strains (H1, H2, H3, H4, H5, H6, 2/3 of H7 strains tested, H8, H9, H10, H11, H12, H14, H15) but not H16.

Structural studies of FluA-20 with the HA head domain revealed a novel epitope that is mostly buried in the peripheral interface of the native HA trimer on the non-receptor-binding site side of the 220-loop, adjacent to the 90-loop1. Many FluA-20 to HA contacts are centered on Arg229, and alanine mutation of Arg229 abolishes binding. Other mutations, at residues Arg220, Val223 or Pro96, also substantially decrease binding. These results are similar in H1, H3, and H5 strains. The key residues recognized by FluA-20, Pro96, Arg220, Pro221, Val223, and Arg229, are highly conserved across diverse subtypes.

Antigen Distribution
HA is on the viral surface.
Background
Hemagglutinin (HA) is a glycoprotein on the Influenza A (IAV) viral surface1. HA consists of two domains: an antigenically variable head and a more conserved stem. There are 18 HA subtypes. Neutralizing antibodies targeting the head domain are typically restricted to within subtype, while antibodies targeting the stem offer broader protection. In contrast, FluA-20 is a human antibody that recognizes the HA head domain of nearly all subtypes of IAV with high affinity1.

Antigen Details

Protein
PubMed
Research Area
Infectious Disease
.
Influenza
.
Viral

References & Citations

1. Bangaru S, Lang S, Schotsaert M, et al. Cell. 177(5):1136-1152.e18. 2019.
Elisa Sandwich Protocol
Flow Cytometry

Formats Available

Prod No.
Description
LT578
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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