Anti-Influenza A, Nucleocapsid

Anti-Influenza A, Nucleocapsid

Product No.: F617

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Product No.F617
Clone
IA-902
Target
Influenza A Nucleocapsid
Product Type
Monoclonal Antibody
Alternate Names
Common flu NP, Influenza A virus NP, NP, Nucleocapsid protein, Nucleoprotein, Protein N, Seasonal Influenza A (H1N1) Nucleocapsid Protein
Isotype
Mouse IgG1
Applications
ELISA
,
IF

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Antibody Details

Product Details

Reactive Species
Influenza A Virus
Host Species
Mouse
Product Concentration
100 μg/ml
Purity
>90% monomer by analytical SEC and SDS-Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline, pH 7.2 and contains 0.1% sodium azide. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
This monoclonal antibody is purified from ascites fluid or culture medium by protein A chromatography or sequential differential precipitations.
Storage and Handling
This product contains no stabilizing proteins and should be stored at 2-8°C until ready to use.
Country of Origin
USA
Shipping
Ships Overnight on Blue Ice
Additional Applications Reported In Literature ?
ELISA Recommended starting range 1:20-1:200
IF Recommended starting range 1:10-1:50
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Anti-Influenza A, Nucleoprotein (Clone IA-902) is specific for the Influenza A nucleocapsid.
Background
There are four types of Influenza (flu) viruses: type A, B, C, and D. Influenza A and B viruses are routinely spread in people and other mammals, while also known for seasonal flu epidemics each year. Influenza A is the genus Alphainfluenzavirus of the virus family Orthomyxoviridae. Influenza A is classified into two main protein subtypes, hemagglutinin (HA) and neuraminidase (NA), located on the surface of the virus. Breaking down the proteins further, there are 18 hemagglutinin subtypes and 11 different neuraminidase subtypes1. Influenza A can be even further classified into specific clades (also known as groups) and sub-clades (also known as sub-groups) based on similarity of their HA/NA gene sequences1. The Nucleocapsid protein or nucleoprotein (NP) of the influenza virus A negative-strand RNA’s primary function is to encapsulate the virus genome for the purpose of RNA transcription, replication and packaging2. Specifically, NP is the most abundant viral protein in infected cells2, therefore the NP can be and has been used for anti-influenza drug development3. The NP of influenza A and B viruses share up to 38% of their amino acid sequence, indicating region functionality differences at the amino acid level4.

Antigen Details

Research Area
Infectious Disease
.
Seasonal and Respiratory Infections
.
IVD Raw Material

References & Citations

1. Types of Influenza viruses (2019) Center for Disease Control and Prevention.
2. Hu Y, Sneyd H, et al. (2017) Curr Top med Chem. 2017; 17(20): 2271–2285.
3. Portela A, Digard P. (2012) J. Gen Virol. 2002 Apr;83(Pt 4):723-734.
4. Sherry L, Smith M, et al. (2014) Journal of Virology. 2014; 88(21); 12326-12338. DOI https://doi.org/10.1128/JVI.01542-14
Indirect Elisa Protocol
IF

Certificate of Analysis

Formats Available

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Prod No.
Description
F617
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.