Anti-Influenza A, Nucleocapsid
Anti-Influenza A, Nucleocapsid
Product No.: F616
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Product No.F616 Clone IA-3808 Target A/Texas Strain ⋅ Influenza A Nucleocapsid Product Type Monoclonal Antibody Alternate Names Common flu NP, Influenza A virus NP, NP, Nucleocapsid protein, Nucleoprotein, Protein N, Seasonal Influenza A (H1N1) Nucleocapsid Protein Isotype Mouse IgG2a Applications ELISA , IF , IHC |
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Antibody DetailsProduct DetailsReactive Species Influenza A Virus Host Species Mouse Product Concentration 100 μg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity >90% monomer by analytical SEC and SDS-Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline, pH 7.2 and contains 0.1% sodium azide. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation This monoclonal antibody is purified from ascites fluid or culture medium by protein A chromatography or sequential differential precipitations. Storage and Handling This product contains no stabilizing proteins and should be stored at 2-8°C until ready to use. Country of Origin USA Shipping Ships Overnight on Blue Ice Additional Applications Reported In Literature ? ELISA Recommended starting range 1:20-1:200 IF Recommended starting range 1:10-1:50 IHC Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Anti-Influenza A, Nucleoprotein (Clone IA-3808) is reactive with >50 separate isolates from H1 through H14. Background There are four types of Influenza (flu) viruses: type A, B, C, and D. Influenza A and B viruses are routinely spread in people and other mammals, while also known for seasonal flu epidemics each year. Influenza A is the genus Alphainfluenzavirus of the virus family Orthomyxoviridae. Influenza A is classified into two main protein subtypes, hemagglutinin (HA) and neuraminidase (NA), located on the surface of the virus. Breaking down the proteins further, there are 18 hemagglutinin subtypes and 11 different neuraminidase subtypes1. Influenza A can be even further classified into specific clades (also known as groups) and sub-clades (also known as sub-groups) based on similarity of their HA/NA gene sequences1. The Nucleocapsid protein or nucleoprotein (NP) of the influenza virus A negative-strand RNA’s primary function is to encapsulate the virus genome for the purpose of RNA transcription, replication and packaging2. Specifically, NP is the most abundant viral protein in infected cells2, therefore the NP can be and has been used for anti-influenza drug development3. The NP of influenza A and B viruses share up to 38% of their amino acid sequence, indicating region functionality differences at the amino acid level4. Antigen DetailsResearch Area Infectious Disease . Seasonal and Respiratory Infections References & Citations1. Types of Influenza viruses (2019) Center for Disease Control and Prevention.
2. Hu Y, Sneyd H, et al. (2017) Curr Top med Chem. 2017; 17(20): 2271–2285. 3. Portela A, Digard P. (2012) J. Gen Virol. 2002 Apr;83(Pt 4):723-734. 4. Sherry L, Smith M, et al. (2014) Journal of Virology. 2014; 88(21); 12326-12338. DOI https://doi.org/10.1128/JVI.01542-14 Technical Protocols IF  |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
