Anti-LCMV nucleoprotein – Purified

Anti-LCMV nucleoprotein – Purified

Product No.: L341

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Clone
VL-4
Target
LCMV Nucleoprotein
Formats AvailableView All
Product Type
Hybridoma Monoclonal Antibody
Alternate Names
Protein N, LCMV Nucleocapsid Protein
Isotype
Rat IgG2a κ
Applications
ELISA
,
FA
,
FC
,
IF

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Select Product Size
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Antibody Details

Product Details

Reactive Species
LCMV
Host Species
Rat
Recommended Isotype Controls
Immunogen
Lymphocytic choriomeningitis virus (LCMV)
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
State of Matter
Liquid
Storage and Handling
This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Regulatory Status
Research Use Only
Country of Origin
USA
Shipping
2-8°C Wet Ice
Additional Applications Reported In Literature ?
IF
N
FA
FC
Focus Formation Assay
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
VL-4 activity is directed against LCMV nucleoprotein, staining LCMV-infected cells internally.
VL-4 does not react with influenza-, vaccinia-, or vesicular stomatitis-infected cells.
Antigen Distribution
LCMV nucleoprotein encapsidates the viral genome RNA and is present in infected cells and virions.
Background
Lymphocytic choriomeningitis virus (LCMV) is a neglected human pathogenic arenavirus with worldwide distribution 1,2. Arenaviruses cause human infection through mucosal exposure to aerosols or by direct contact with abraded skin of infected rodents 1. LCMV infection may carry health risks for humans who are immunocompromised or pregnant 2.

LCMV infection in mice can be acute or persistent depending on age, immunocompetence, genetic background, route of infection, strain, and dosage 1. Due to the versatility of outcome, LCMV mouse models are extensively used to examine basic questions of immunology and virology, including: virus-induced immunopathological disease, MHC restriction, T cell and B cell regulation, T cell-mediated killing, and immune T cell therapy in clearing viral infection 1,2. LCMV mouse models were used to identify PD-1 as a critical regulator of T cell exhaustion 2 and have also been used to study the synergy between PD-1 blockade and IL-2 cytokine in cancer immunotherapy 3.

LCMV is an enveloped virus with a bi-segmented negative-stranded, ambisense RNA genome 1,2. LCMV has a non-cytolytic life cycle restricted to the cell cytoplasm. Cell entry is cholesterol-dependent but clathrin-, dynamin-, caveolin-, ARF6-, flotillin-, and actin-independent and occurs via receptor-mediated endocytosis utilizing alpha-dystroglycan as the main extracellular matrix protein receptor 1. Most of the disease caused by LCMV is mediated by the host T cell response 2.

Arenavirus nucleoprotein (NP) is the most abundant viral protein component in virions as well as in infected cells 4. NP encapsidates the viral genomic RNA and is part of the viral ribonucleoprotein complex that directs viral RNA replication and gene transcription in the cytoplasm of infected cells. NP also counteracts host type I interferon response during infection via a functional 3’–5’ exonuclease domain in its C-terminal region 5,6. The same domain also interacts with LCMV Z, as well as Lassa Virus Z, but different residues are involved 4. This NP-Z interaction is a novel target for antiviral drug development.

VL-4 antibody was generated by immunizing a (Louvain X DA) F1 rat with LCMV strain WE and fusing the resulting spleen cells with the YM3 myeloma cell line 7.

Antigen Details

UniProt.org
Research Area
Infectious Disease

References & Citations

1. Grande-Pérez A, Martin V, Moreno H, et al. Curr Top Microbiol Immunol. 392:231-276. 2016.
2. Dangi T, Chung YR, Palacio N, et al. Curr Protoc Immunol. 130(1):e99. 2020.
3. Hashimoto M, Araki K, Cardenas MA, et al. Nature. 610(7930):173-181. 2022.
4. Ortiz-Riaño E, Cheng BY, de la Torre JC, et al. J Virol. 85(24):13038-13048. 2011.
5. Martínez-Sobrido L, Zúñiga EI, Rosario D, et al. J Virol. 80: 9192–9199. 2006.
6. Borrow P, Martinez-Sobrido L, de la Torre JC. Viruses 2: 2443–2480. 2010.
7. Battegay M, Cooper S, Althage A, et al. J Virol Methods. 33(1-2):191-198. 1991.
8. Seiler P, Kalinke U, Rülicke T, et al. J Virol. 72(3):2253-2258. 1998.
9. Straub T, Schweier O, Bruns M, et al. Eur J Immunol. 43(9):2338-2348. 2013.
Indirect Elisa Protocol
FA
Flow Cytometry
IF
Products are for research use only. Not for use in diagnostic or therapeutic procedures.